liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Molecular gradients: An efficient approach for optimizing the surface properties of biomaterials and biochips
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Sensor Science and Molecular Physics .
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Applied Physics .
Show others and affiliations
2005 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 21, no 3, p. 1042-1050Article in journal (Refereed) Published
Abstract [en]

A variety of molecular gradients of alkanethiols with the structure HS-(CH2)m-X (m = 15, X = COOH, CH2NH 2, or CH3) and oligo(ethylene glycol)-terminated alkanethiols with the structures HS-(CH2)15-CO-NH-Eg n (n = 2, 4, or 6), HS-(CH2)15-CO-NH-Eg 2-(CH2)2-NH-CO-(CH2) 4-biotin, and HS-(CH2)15-CO-NH-Eg 6-CH2-COOH were prepared on polycrystalline gold films. These gradients were designed to serve as model surfaces for fundamental studies of protein adsorption and immobilization phenomena. Ellipsometry, infrared spectroscopy, and X-ray photoelectron spectroscopy, operating in scanning mode, were used to monitor the layer composition, gradient profiles, tail group conformation, and overall structural quality of the gradient assemblies. The gradient profiles were found to be 4-10 mm wide, and they increased in width with increasing difference in molecular complexity between the thiols used to form the gradient. The oligo(ethylene glycol) thiols are particularly interesting because they can be used to prepare so-called conformational gradients, that is, gradients that display a variation in oligo(ethylene glycol) chain conformation from all trans on the extreme Eg 2,4 sides, via an amorphous-like phase in the mixing regimes, to helical at the extreme Eg6 sides. We demonstrate herein a series of experiments where the above gradients are used to evaluate nonspecific binding of the plasma protein fibrinogen, and in agreement with previous studies, the highest amounts of nonspecifically bound fibrinogen were observed on all-trans monolayers, that is, on the extreme Eg2,4 sides. Moreover, gradients between Eg2 and a biotinylated analogue have been prepared to optimize the conditions for the immobilization of streptavidin. Ellipsometry and infrared spectroscopy reveal high levels of immobilization over a fairly broad range of compositions in the gradient regime, with a maximum between 50 and 60% of the biotinylated analogue in the monolayer. A pi gradient composed of (NH3+/COO-)-terminated thiols was also prepared and evaluated with respect to its ability to separate differently charged proteins, pepsin, and lysozyme, on a solid surface.

Place, publisher, year, edition, pages
2005. Vol. 21, no 3, p. 1042-1050
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-45519DOI: 10.1021/la048358mOAI: oai:DiVA.org:liu-45519DiVA, id: diva2:266415
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2017-12-13

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full text

Authority records

Östblom, MattiasLundström, IngemarLiedberg, Bo

Search in DiVA

By author/editor
Östblom, MattiasLundström, IngemarLiedberg, Bo
By organisation
The Institute of TechnologySensor Science and Molecular Physics Applied Physics
In the same journal
Langmuir
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 176 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf