Optical emission of a conjugated polyelectrolyte: calcium-induced conformational changes in calmodulin and calmodulin-calcineurin interactions
2004 (English)In: Macromolecules, ISSN 0024-9297, E-ISSN 1520-5835, Vol. 37, no 24, 9109-9113 p.Article in journal (Refereed) Published
Electronic polymers in aqueous media offer bioelectronic detection of biomolecular processes. Here we report fluorometric detection of calcium-induced conformational changes in calmodulin based on noncovalent assembly of calmodulin to a water-soluble zwitterionic polythiophene derivative. Assembly with calmodulin will induce a planar geometry and aggregation of the polymer chains, detected as a decrease of the intensity and a red shift of the fluorescence. Upon addition of Ca2+ the intensity of the emitted light is increased and blue-shifted. The geometrical alteration of the polymer chains can further be utilized for recording of the binding of calcineurin to the calcium-activated POWT−calmodulin complex. This novel methodology, using a conformation-sensitive probe, allows fluorometric detection of conformational changes in biomolecules and protein−protein interactions without any covalent modifications of the biomolecules. The rapid and selective method is based on noncovalent interactions between a zwitterionic polythiophene derivative and the biomolecule of interest. This offers a novel way to create microarrays without using covalent attachment of the receptor or labeling of the analyte.
Place, publisher, year, edition, pages
2004. Vol. 37, no 24, 9109-9113 p.
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-45577DOI: 10.1021/ma048605tOAI: oai:DiVA.org:liu-45577DiVA: diva2:266473