liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Specification of Drosophila motoneuron identity by the combinatorial action of POU and LIM-HD factors
Department of Neurobiology, Harvard Medical School, 220 Longwood Avenue, Boston, MA 02115, United States.
Linköping University, Department of Physics, Chemistry and Biology, Biology. Linköping University, The Institute of Technology.
2004 (English)In: Development, ISSN 0950-1991, E-ISSN 1477-9129, Vol. 131, no 21, 5429-5439 p.Article in journal (Refereed) Published
Abstract [en]

In both vertebrates and invertebrates, members of the LIM-homeodomain (LIM-HD) family of transcription factors act in combinatorial codes to specify motoneuron subclass identities. In the developing Drosophila embryo, the LIM-HD factors Islet (Tailup) and Lim3, specify the set of motoneuron subclasses that innervate ventral muscle targets. However, as several subclasses express both Islet and Lim3, this combinatorial code alone cannot explain how these motoneuron groups are further differentiated. To identify additional factors that may act to refine this LIM-HD code, we have analyzed the expression of POU genes in the Drosophila embryonic nerve cord. We find that the class III POU protein, Drifter (Ventral veinless), is co-expressed with Islet and Lim3 specifically in the ISNb motoneuron subclass. Loss-of-function and misexpression studies demonstrate that the LIM-HD combinatorial code requires Drifter to confer target specificity between the ISNb and TN motoneuron subclasses. To begin to elucidate molecules downstream of the LIM-HD code, we examined the involvement of the Beaten path (Beat) family of immunoglobulin-containing cell-adhesion molecules. We find that beat Ic genetically interacts with islet and Lim3 in the TN motoneuron subclass and can also rescue the TN fasciculation defects observed in islet and Lim3 mutants. These results suggest that in the TN motoneuron context, Islet and Lim3 may specify axon target selection through the actions of IgSF call-adhesion molecules.

Place, publisher, year, edition, pages
2004. Vol. 131, no 21, 5429-5439 p.
Keyword [en]
Combinatorial code, Drifter, Drosophila, Islet, Lim3, Motoneutrons
National Category
Natural Sciences
URN: urn:nbn:se:liu:diva-45582DOI: 10.1242/dev.01418OAI: diva2:266478
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2012-01-07

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Thor, Stefan
By organisation
BiologyThe Institute of Technology
In the same journal
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 48 hits
ReferencesLink to record
Permanent link

Direct link