liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Silane-dextran chemistry on lateral flow polymer chips for immunoassays
Jönsson, C., Åmic AB, Uppsala, Sweden, Biomedical Diagnostics Institute, NCSR Building, Dublin City University, Glasnevin Dublin 9, Ireland.
Åmic AB, Uppsala, Sweden.
Rundström, G., Åmic AB, Uppsala, Sweden.
Åmic AB, Uppsala, Sweden.
Show others and affiliations
2008 (English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 8, no 7, 1191-1197 p.Article in journal (Refereed) Published
Abstract [en]

The prognosis for patients suffering from cardiovascular and many other diseases can be substantially improved if diagnosed at an early stage. High performance diagnostic testing using disposable microfluidic chips can provide a platform for realizing this vision. Åmic AB (Uppsala, Sweden) has developed a new microfluidic test chip for sandwich immunoassays fabricated by injection molding of the cycloolefin-copolymer Zeonor™. A highly ordered array of micropillars within the fluidic chip distributes the sample solution by capillary action. Since wetting of the pillar array surface is the only driving force for liquid distribution precise control of the surface chemistry is crucial. In this work we demonstrate a novel protocol for surface hydrophilization and antibody immobilization on cycloolefin-copolymer test chips, based on direct silanisation of the thermoplastic substrate. Dextran is subsequently covalently coupled to amino groups, thus providing a coating with a low contact angle suitable for antibody immobilization. The contact angle of dextran coated chips is stable for at least two months, which enables production of large batches that can be stored for extended periods of time. We demonstrate the utility of the presented platform and surface chemistry in a C-reactive protein assay with a detection limit of 2.6 ng ml-1, a dynamic range of 102 and a coefficient of variance of 15%. © The Royal Society of Chemistry.

Place, publisher, year, edition, pages
2008. Vol. 8, no 7, 1191-1197 p.
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-45778DOI: 10.1039/b800297eOAI: diva2:266674
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2013-02-27

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Martinsson, ErikLiedberg, Bo
By organisation
Sensor Science and Molecular PhysicsThe Institute of Technology
In the same journal
Lab on a Chip
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 64 hits
ReferencesLink to record
Permanent link

Direct link