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Innervation of tissue-engineered recombinant human collagen-based corneal substitutes: A comparative in vivo confocal microscopy study
University of Ottawa Eye Institute.
Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON, Canada.ORCID iD: 0000-0003-1222-6720
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Ophthalmology. Östergötlands Läns Landsting, Reconstruction Centre, Department of Ophthalmology UHL/MH.
University of Ottawa Eye Institute.
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2008 (English)In: Investigative Ophthalmology and Visual Science, ISSN 0146-0404, E-ISSN 1552-5783, Vol. 49, no 9, 3895-3902 p.Article in journal (Refereed) Published
Abstract [en]

PURPOSE. To compare reinnervation in recombinant human collagen-based corneal substitutes with allografts during a 1-year postimplantation follow-up period in pigs. A retrospective comparison to innervation in porcine collagen-based biosynthetic grafts was also performed. METHODS. Pigs received a corneal allograft or a substitute made of either recombinant human type-I or -III collagen. In vivo confocal microscopic examination of the central cornea of surgical and untouched control eyes before surgery and at 2, 6, and 12 months after surgery was performed to quantify the number, density, and diameter of nerves at various corneal depths. RESULTS. By 12 months after surgery, the number and density of regenerated nerves in the anterior and deep anterior corneal stroma recovered to preoperative and control levels in both types of substitute grafts and in the allografts. In the subepithelial and subbasal regions, however, significantly fewer nerves were detected relative to those in control subjects at 12 months, regardless of graft type ( P < 0.05), similar to the behavior of porcine collagen-based biosynthetic grafts. An absence of thick stromal nerve trunks (diameter, > 10 mu m) in all grafts, irrespective of material type, indicated that nerve regeneration in grafts was accompanied by persistent morphologic changes. CONCLUSIONS. Nerve regeneration in recombinant human collagen-based biosynthetic corneal grafts proceeded similarly to that in allograft tissue, demonstrating the suitability of recombinant human collagen constructs as nerve-friendly corneal substitutes. Furthermore, only minor differences were noted between type-I and -III collagen grafts, indicating an insensitivity of nerve regeneration to initial collagen type.

Place, publisher, year, edition, pages
2008. Vol. 49, no 9, 3895-3902 p.
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Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-45863DOI: 10.1167/iovs.07-1354OAI: oai:DiVA.org:liu-45863DiVA: diva2:266759
Note
Original Publication: Neil Lagali, May Griffith, Per Fagerholm, Kimberley Merrett, Melissa Huynh and Rejean Munger, Innervation of tissue-engineered recombinant human collagen-based corneal substitutes: A comparative in vivo confocal microscopy study, 2008, Investigative Ophthalmology and Visual Science, (49), 9, 3895-3902. http://dx.doi.org/10.1167/iovs.07-1354 Copyright: Research in Vision and Opthalmology http://www.arvo.org/ Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2017-12-13

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