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Conserved epitopes in human and mouse tissue-nonspecific alkaline phosphatase - Second report of the ISOBM TD-9 Workshop
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Östergötlands Läns Landsting, Center for Diagnostics, Department of Clinical Chemistry.
2005 (English)In: Tumor Biology, ISSN 1010-4283, E-ISSN 1423-0380, Vol. 26, no 3, 113-120 p.Article in journal (Refereed) Published
Abstract [en]

A panel of 19 monoclonal antibodies (MAbs) against human tissue-nonspecific (liver/bone/kidney) alkaline phosphatase (TNAP) was obtained through the ISOBM TD-9 workshop. In the present study, the reactivity of these MAbs has been characterized against mouse TNAP. A mouse embryonic stem cell line, frozen sections of long bones, alkaline phosphatase extracted from mouse bone, and serum were used as the source of TNAP for individual assays. Each MAb was tested for immunoreactivity to mouse TNAP by Western blot analysis, immunohistochemistry and enzyme immunoassay. Antibodies 314 and 315 reacted strongly with mouse TNAP in Western blots, while all other antibodies were negative. By immunohistochemistry, antibodies 314, 315 and 333 produced strong positive staining using frozen sections, while antibody 334 was moderately positive. Enzyme immunoassays indicated that MAb 333 was also able to bind to serum TNAP. These antibodies represent very useful reagents to study the pathophysiological expression of TNAP in mouse tissues and in mouse serum. Copyright (C) 2005 S. Karger AG, Basel.

Place, publisher, year, edition, pages
2005. Vol. 26, no 3, 113-120 p.
Keyword [en]
alkaline phosphatase, bone, epitope specificity, Western blot, immunohistochemistry, immunoassays, monoclonal antibodies
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-46101DOI: 10.1159/000086482OAI: diva2:266997
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2013-10-18

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Magnusson, Per
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Faculty of Health SciencesClinical ChemistryDepartment of Clinical Chemistry
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