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Peptide functionalized poly(L-lysine)-g-poly(ethylene glycol) on titanium: Resistance to protein adsorption in full heparinized human blood plasma
Lab. for Surf. Sci. and Technology, Department of Materials, Swiss Fed. Inst. Technol. (ETH) Z., CH-8092 Zurich, Switzerland.
De Paul, S.M., Lab. for Surf. Sci. and Technology, Department of Materials, Swiss Fed. Inst. Technol. (ETH) Z., CH-8092 Zurich, Switzerland, Solvias AG, Klybeckstrasse 191, CH-4002 Basel, Switzerland.
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology.
Institute for Biomedical Engineering, Department of Materials, ETH and University of Zurich, CH-8092 Zurich, Switzerland.
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2003 (English)In: Biomaterials, ISSN 0142-9612, Vol. 24, no 27, 4949-4958 p.Article in journal (Refereed) Published
Abstract [en]

The graft copolymer poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) and its RGD- and RDG-functionalized derivatives (PLL-g-PEG/PEG-peptide) were assembled from aqueous solutions on titanium (oxide) surfaces. The polymers were characterized by NMR in order to determine quantitatively the grafting ratio, g (Lys monomer units/PEG side chains), and the fraction of the PEG side chains carrying the terminal peptide group. The titanium surfaces modified with the polymeric monomolecular adlayers were exposed to full heparinized blood plasma. The adsorbed masses were measured by in situ ellipsometry. The different PLL-g-PEG-coated surfaces showed, within the detection limit of the ellipsometric technique, no statistically significant protein adsorption during exposure to plasma for 30min at 22°C or 37°C, whereas clean, uncoated titanium surfaces adsorbed approximately 350ng/cm2 of plasma proteins. The high degree of resistance of the PEGylated surface to non-specific adsorption makes peptide-modified PLL-g-PEG a useful candidate for the surface modification of biomedical devices such as implants that are capable of eliciting specific interactions with integrin-type cell receptors even in the presence of full blood plasma. The results refer to short-term blood plasma exposure that cannot be extrapolated a priori to long-term clinical performance. © 2003 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
2003. Vol. 24, no 27, 4949-4958 p.
Keyword [en]
Blood plasma, Graft copolymers, Peptide, Poly(ethylene glycol), Protein resistance, Titanium
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-46396DOI: 10.1016/S0142-9612(03)00420-4OAI: diva2:267292
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2011-01-13

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