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Improved selectivity of microbial biosensor using membrane coating. Application to the analysis of ethanol during fermentation
Department of Biochemical Technology, Fac. of Chemical and Food Technology, Slovak University of Technology, Radlinskeho 9, SK-812 37 Bratislava, Slovakia.
Department of Analytical Chemistry, Lund University, PO Box 124, SE-22 100 Lund, Sweden.
Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, SK-842 38 Bratislava, Slovakia.
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2003 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, Vol. 18, no 9, 1125-1134 p.Article in journal (Refereed) Published
Abstract [en]

A ferricyanide mediated microbial biosensor for ethanol detection was prepared by surface modification of a glassy carbon electrode. The selectivity of the whole Gluconobacter oxydans cell biosensor for ethanol determination was greatly enhanced by the size exclusion effect of a cellulose acetate (CA) membrane. The use of a CA membrane increased the ethanol to glucose sensitivity ratio by a factor of 58.2 and even the ethanol to glycerol sensitivity ratio by a factor of 7.5 compared with the use of a dialysis membrane. The biosensor provides rapid and sensitive detection of ethanol with a limit of detection of 0.85 µM (S/N=3). The selectivity of the biosensor toward alcohols was better compared to previously published enzyme biosensors based on alcohol oxidase or alcohol dehydrogenases. The biosensor was successfully used in an off-line monitoring of ethanol during batch fermentation by immobilized Saccharomyces cerevisiae cells with an initial glucose concentration of 200 g l-1. © 2002 Elsevier Science B.V. All rights reserved.

Place, publisher, year, edition, pages
2003. Vol. 18, no 9, 1125-1134 p.
Keyword [en]
Cellulose acetate, Enhanced selectivity, Ethanol detection, Ferricyanide, Gluconobacter oxydans, Microbial biosensor
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-46522DOI: 10.1016/S0956-5663(02)00244-0OAI: oai:DiVA.org:liu-46522DiVA: diva2:267418
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2011-01-13

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