Improved selectivity of microbial biosensor using membrane coating. Application to the analysis of ethanol during fermentation
2003 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, Vol. 18, no 9, 1125-1134 p.Article in journal (Refereed) Published
A ferricyanide mediated microbial biosensor for ethanol detection was prepared by surface modification of a glassy carbon electrode. The selectivity of the whole Gluconobacter oxydans cell biosensor for ethanol determination was greatly enhanced by the size exclusion effect of a cellulose acetate (CA) membrane. The use of a CA membrane increased the ethanol to glucose sensitivity ratio by a factor of 58.2 and even the ethanol to glycerol sensitivity ratio by a factor of 7.5 compared with the use of a dialysis membrane. The biosensor provides rapid and sensitive detection of ethanol with a limit of detection of 0.85 µM (S/N=3). The selectivity of the biosensor toward alcohols was better compared to previously published enzyme biosensors based on alcohol oxidase or alcohol dehydrogenases. The biosensor was successfully used in an off-line monitoring of ethanol during batch fermentation by immobilized Saccharomyces cerevisiae cells with an initial glucose concentration of 200 g l-1. © 2002 Elsevier Science B.V. All rights reserved.
Place, publisher, year, edition, pages
2003. Vol. 18, no 9, 1125-1134 p.
Cellulose acetate, Enhanced selectivity, Ethanol detection, Ferricyanide, Gluconobacter oxydans, Microbial biosensor
IdentifiersURN: urn:nbn:se:liu:diva-46522DOI: 10.1016/S0956-5663(02)00244-0OAI: oai:DiVA.org:liu-46522DiVA: diva2:267418