liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Preparation of multilayer plasma protein films on silicon by EDC/NHS coupling chemistry
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Applied Physics .
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology.
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology.
Inst. of Anatomy and Cell Biology, Göteborg University, P.O. Box 420, SE-405 30 Gotebörg, Sweden.
Show others and affiliations
2003 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 28, no 4, 261-272 p.Article in journal (Refereed) Published
Abstract [en]

Crosslinked multilayer protein films were prepared from fibrinogen, albumin, IgG, a combination of fibrinogen and catalase, and blood plasma on silicon by ethyl-dimethyl-aminopropylcarbodiimide and N-hydroxy-succinimide coupling chemistry. The 4-70 nm thick films were placed in blood plasma and the additional protein deposition measured by null ellipsometry after 5 or 60 min of incubation. The activation of the complement system and intrinsic pathway of coagulation were indicated through the subsequent binding of anti-C3c, anti-C3d, anti-properdin and anti-HMWK on top of the surface bound blood plasma. The proportion of Annexin V, Propidium Iodide and 4,6-diamidino-2-phenylindole positive cells, and the secretion of tumor necrosis factor a (TNF-a) and interleukin-10 (IL-10) were analysed in a monocyte culture. The results show that well known protein coupling techniques can be used for the preparation of protein layers with well controlled thickness. The layers possess low contact activation of blood plasma and induce different release of TNF-a and IL-10 in monocyte cultures. © 2002 Elsevier Science B.V. All rights reserved.

Place, publisher, year, edition, pages
2003. Vol. 28, no 4, 261-272 p.
Keyword [en]
Adsorption, Cytokine, Monocyte, Multilayer, Protein
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-46560DOI: 10.1016/S0927-7765(02)00151-0OAI: oai:DiVA.org:liu-46560DiVA: diva2:267456
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2017-12-13

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Tengvall, PenttiJansson, EvaAskendal, Agneta

Search in DiVA

By author/editor
Tengvall, PenttiJansson, EvaAskendal, Agneta
By organisation
The Institute of TechnologyApplied Physics Department of Physics, Chemistry and Biology
In the same journal
Colloids and Surfaces B: Biointerfaces
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 115 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf