Adsorption of human serum albumin in porous silicon gradients
2003 (English)Conference paper (Other academic)
Backside etching has been utilized to produce gradients of pore size and layer thickness in porous silicon. Human serum albumin (HSA) was adsorbed on such gradients at two different pH values: 4.9, the pI of HSA, and 7.4, the physiological pH. The samples were investigated by scanning electron microscopy, spectroscopic ellipsometry, and autoradiography. The results show that the protein adsorbed displays a gradient along with the pore size and the thickness gradient. The higher than current density used during etching, the more sway-back shaped curves were seen for the protein adsorption pattern, independent of pH. When 50 mA/cm2 current density was used during etching, the quota between the maximal intensity value and the plateau value seen after adsorption of the HSA increased for pH 7.4.
Place, publisher, year, edition, pages
2003. Vol. 197, no 2, 326-330 p.
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-46654DOI: 10.1002/pssa.200306518OAI: oai:DiVA.org:liu-46654DiVA: diva2:267550