A mesoscopic network in the form of a hydrogel of the highly conductive polymer PEDOT/PSS hydrogel was used in an enzyme electrode setup. Osmium was used both as a crosslink point in the hydrogel network and as a mediator between the prosthetic group of the enzyme and the conductive polymer matrix. Both biostability and high conductivity is important aspects when building nerve- or cell- electrodes. Diffusion of analytes surrounding the cells into the matrix electrode is feasible due to the open hydrogel structure. The high water content in these structures is important when buffering them to a pH of choice.