liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
RelA/NF-?B transcription factor associates with a-actinin-4
Department of Cell Cultures, Institute of Cytology, Tikhoretsky Ave. 4, St. Petersburg, 194064, Russian Federation.
Department of Cell Cultures, Institute of Cytology, Tikhoretsky Ave. 4, St. Petersburg, 194064, Russian Federation.
Department of Cell Cultures, Institute of Cytology, Tikhoretsky Ave. 4, St. Petersburg, 194064, Russian Federation.
Department of Cell Cultures, Institute of Cytology, Tikhoretsky Ave. 4, St. Petersburg, 194064, Russian Federation.
Show others and affiliations
2008 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 314, no 5, 1030-1038 p.Article in journal (Refereed) Published
Abstract [en]

The NF-?B/RelA family of transcription factors regulates inducible transcription of a large number of genes in response to diverse stimuli. Little is known, however, about the location of NF-?B in the cytoplasm and the transport mechanism to the nucleus. We found that NF-?B is associated with the actin-binding protein a-actinin-4. NF-?B and a-actinin-4 co-localized along actin stress fibers and in membrane lamellae in A431 cells. After a 30-min stimulation with EGF or TNF-a, a-actinin-4 and p65 were found in the nucleus. Disruption of cytoskeleton by cytochalasin D prior to treatment with TNF-a led to increase of p65 nuclear translocation. Antibodies to p65 subunit of NF-?B co-immunoprecipitated a-actinin-4 from A431 cell lysates and nuclear extracts, but a-actinin-1 and ß-actin were not found in the precipitates. Affinity chromatography experiments displayed that p65 and p50 subunits of NF-?B can bind to matrix-bound chicken gizzard a-actinin. We suggest that the a-actinin-4 is important for the NF-?B nuclear translocation and its functions inside the nucleus. © 2007 Elsevier Inc. All rights reserved.

Place, publisher, year, edition, pages
2008. Vol. 314, no 5, 1030-1038 p.
Keyword [en]
a-Actinin-1, a-Actinin-4, Actin cytoskeleton, NF-?B, p65, RelA
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-46678DOI: 10.1016/j.yexcr.2007.12.001OAI: oai:DiVA.org:liu-46678DiVA: diva2:267574
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2017-12-13

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Magnusson, Karl-Eric

Search in DiVA

By author/editor
Magnusson, Karl-Eric
By organisation
Faculty of Health SciencesMedical Microbiology
In the same journal
Experimental Cell Research
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 66 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf