RelA/NF-?B transcription factor associates with a-actinin-4
2008 (English)In: Experimental Cell Research, ISSN 0014-4827, Vol. 314, no 5, 1030-1038 p.Article in journal (Refereed) Published
The NF-?B/RelA family of transcription factors regulates inducible transcription of a large number of genes in response to diverse stimuli. Little is known, however, about the location of NF-?B in the cytoplasm and the transport mechanism to the nucleus. We found that NF-?B is associated with the actin-binding protein a-actinin-4. NF-?B and a-actinin-4 co-localized along actin stress fibers and in membrane lamellae in A431 cells. After a 30-min stimulation with EGF or TNF-a, a-actinin-4 and p65 were found in the nucleus. Disruption of cytoskeleton by cytochalasin D prior to treatment with TNF-a led to increase of p65 nuclear translocation. Antibodies to p65 subunit of NF-?B co-immunoprecipitated a-actinin-4 from A431 cell lysates and nuclear extracts, but a-actinin-1 and ß-actin were not found in the precipitates. Affinity chromatography experiments displayed that p65 and p50 subunits of NF-?B can bind to matrix-bound chicken gizzard a-actinin. We suggest that the a-actinin-4 is important for the NF-?B nuclear translocation and its functions inside the nucleus. © 2007 Elsevier Inc. All rights reserved.
Place, publisher, year, edition, pages
2008. Vol. 314, no 5, 1030-1038 p.
a-Actinin-1, a-Actinin-4, Actin cytoskeleton, NF-?B, p65, RelA
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-46678DOI: 10.1016/j.yexcr.2007.12.001OAI: oai:DiVA.org:liu-46678DiVA: diva2:267574