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The determination of thickness and surface mass density of mesothick immunoprecipitate layers by null ellipsometry and protein 125Iodine labeling
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology.
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology.
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Applied Physics .
2002 (English)In: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 249, no 1, 84-90 p.Article in journal (Refereed) Published
Abstract [en]

The aim of the present study was to ellipsometrically determine the thickness and surface mass density in air for up to 110-nm-thick organic layers made of alternatingly deposited layers of HSA and polyclonal anti-HSA on hydrophobic silicon. The ellipsometrically determined thickness was compared to that obtained by AFM and the deposited surface mass density calibrated with 125I-labeled proteins. The results indicate a good agreement in protein layer thickness between AFM and ellipsometry when the protein film refractive index Nfilm = 1.5 -0i, although then the calculated surface mass density from the ellipsometry data became grossly overestimated by the Cuypers one-component formula. A good agreement in the surface mass density was obtained when the M/A ratio in this formula was lowered from 4.14 to 2.35. This approach indicates a convenient means of determining the refractive indices and surface mass densities of mesothick organic layers proteins on solid supports. © 2002 Elsevier Science (USA).

Place, publisher, year, edition, pages
2002. Vol. 249, no 1, 84-90 p.
Keyword [en]
Ellipsometry, Mass, Protein adsorption, Radio labeling, Thickness
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-46903DOI: 10.1006/jcis.2002.8247OAI: oai:DiVA.org:liu-46903DiVA: diva2:267799
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2017-12-13

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Benesch, JohanAskendal, AgnetaTengvall, Pentti

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