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The importance of being knotted: Effects of the C-terminal knot structure on enzymatic and mechanical properties of bovine carbonic anhydrase II1
Laboratory of Biodynamics, Grad. Sch. of Biosci. and Biotech., Tokyo Inst. of Technol., 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Laboratory of Biodynamics, Grad. Sch. of Biosci. and Biotech., Tokyo Inst. of Technol., 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Biochemistry.
Laboratory of Biodynamics, Grad. Sch. of Biosci. and Biotech., Tokyo Inst. of Technol., 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
2002 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 519, no 1-3, 35-40 p.Article in journal (Refereed) Published
Abstract [en]

In order to better understand the contribution of the knotted folding pattern to the enzymatic and mechanical properties of carbonic anhydrases, we replaced Gln-253 of bovine carbonic anhydrase II with Cys, which allowed us to measure the mechanical strength of the protein against tensile deformation by avoiding knot tightening. The expressed protein, to our surprise, turned out to contain two conformational isomers, one capable of binding an enzymatic inhibitor and the other not, which led to their separation through affinity chromatography. In near- and far-UV circular dichroism and fluorescence spectra, the separated conformers were very similar to each other and to the wild-type enzyme, indicating that they both had native-like conformations. We describe new evidence which supports the notion that the difference between the two conformers is likely to be related to the completeness of the C-terminal knot formation. © 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

Place, publisher, year, edition, pages
2002. Vol. 519, no 1-3, 35-40 p.
Keyword [en]
Atomic force microscope, Carbonic anhydrase II, Conformer, Knot structure, Mechanical unfolding
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-47002DOI: 10.1016/S0014-5793(02)02693-5OAI: oai:DiVA.org:liu-47002DiVA: diva2:267898
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2017-12-13

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Carlsson, Uno

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