Ex vivo PMA-induced respiratory burst and TNF-a secretion elicited from inflammatory cells on machined and porous blood plasma clot-coated titanium
2002 (English)In: Biomaterials, ISSN 0142-9612, Vol. 23, no 13, 2803-2815 p.Article in journal (Refereed) Published
The release of inflammatory mediators around implants and normal wounds may differ due to the presence of the solid surface. In this study, machined and sub-micron porous titanium implants with and without a 100nm thick blood plasma clot were inserted subcutaneously in rat for 3 or 24h. The cell recruitment to the interfaces, in vivo secretion of TNF-a and the ex vivo PMA-induced production of reactive oxygen species were subsequently investigated. The thin plasma clot coating gave rise to an increased ex vivo PMA-stimulated oxygen radical production by implant-associated cells at both implantation times, and an increased cell recruitment at 24h. The total TNF-a secretion was highest at sham sites and plasma clot-coated porous titanium at 24h. After 24h, the cell-type pattern in the exudate around the porous plasma-coated implant was more similar to that found at sham sites than that adjacent to the non-coated implants. No differences were observed between the machined Ti and the machined sub-micron porous Ti. © 2002 Elsevier Science Ltd. All rights reserved.
Place, publisher, year, edition, pages
2002. Vol. 23, no 13, 2803-2815 p.
Coagulation, Fibrinogen, Inflammation, Plasma, Porous surfaces
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-47012DOI: 10.1016/S0142-9612(02)00021-2OAI: oai:DiVA.org:liu-47012DiVA: diva2:267908