Simultaneous analysis of ?-hydroxybutyric acid, ?-butyrolactone, and 1,4-butanediol by micellar electrokinetic chromatography
2002 (English)In: Forensic Science International, ISSN 0379-0738, Vol. 125, no 2-3, 113-119 p.Article in journal (Refereed) Published
A micellar electrokinetic chromatography (MEKC) method was optimised for simultaneous analysis of ?-hydroxybutyric acid (GHB), ?-butyrolactone (GBL), and 1,4-butanediol (BD). Best conditions for separation and baseline stability were achieved using a carrier electrolyte comprising 30.0mM sodium barbital and 150.0mM sodium dodecyl sulphate (SDS) at pH 10.2. Calibration functions were linear, giving correlation coefficients (r2) >0.998 for the three target compounds. Limits of detection (LOD) defined as three times the noise, were 5.1mg/l, 0.34 and 0.25g/l for GHB, GBL and BD, respectively. The repeatability of migration times and peak areas, expressed as the R.S.D. (n=9) was better than 0.41 and 3.05%, respectively. Some casework samples were analysed using the optimised conditions. Copyright © 2002 Elsevier Science Ireland Ltd.
Place, publisher, year, edition, pages
2002. Vol. 125, no 2-3, 113-119 p.
?-Butyrolactone, ?-Hydroxybutyric acid, 1, 4-Butanediol, Capillary electrophoresis, Micellar electrokinetic chromatography
IdentifiersURN: urn:nbn:se:liu:diva-47089DOI: 10.1016/S0379-0738(02)00006-3OAI: oai:DiVA.org:liu-47089DiVA: diva2:267985