liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Stabilizing plasmid copy number to improve recombinant protein production
Institute of Applied Microbiology, University of Agricultural Science, Vienna, Muthgasse 18, A-1190 Vienna, Austria.
Linköping University, The Institute of Technology.
Institute of Applied Microbiology, University of Agricultural Science, Vienna, Muthgasse 18, A-1190 Vienna, Austria.
Institute of Applied Microbiology, University of Agricultural Science, Vienna, Muthgasse 18, A-1190 Vienna, Austria.
2002 (English)In: Biotechnology and Bioengineering, ISSN 0006-3592, E-ISSN 1097-0290, Vol. 77, no 2, 142-147 p.Article in journal (Refereed) Published
Abstract [en]

The key objective for recombinant protein production in bacteria is the maximum exploitation of the cell factory's potential, whereby often strong expression vectors are used to increase product yield. If the metabolic load caused by recombinant expression exceeds the host's capacity, the system exhausts itself, resulting in a loss of protein yield. Excessive plasmid replication is observed after inducing recombinant gene expression, which greatly contributes to metabolic overload of the host cell. The transcriptional and translational machineries are extremely overstrained. By abolishing sequence homology between ColE1 RNA I/RNA II and tRNAs, we were able to restore the plasmid's replication control mechanisms and to keep the plasmid copy number constant throughout the culture process, thereby prolonging metabolic activity and productivity of the bacterial expression system. Because the bacterial host cell is not being exploited beyond its tolerable potential with this method, the constancy of the plasmid copy number level throughout the whole period of the bioprocess provides novel strategies for bioprocess optimization. © 2002 John Wiley & Sons, Inc.

Place, publisher, year, edition, pages
2002. Vol. 77, no 2, 142-147 p.
Keyword [en]
Escherichia coli, Plasmid Co/E1, Recombinant, Replication, RNA I/RNA II
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-47125DOI: 10.1002/bit.10104OAI: oai:DiVA.org:liu-47125DiVA: diva2:268021
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2012-01-07

Open Access in DiVA

No full text

Other links

Publisher's full text
By organisation
The Institute of Technology
In the same journal
Biotechnology and Bioengineering
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 18 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf