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Distribution and Function of the Hydrogen Sulfide-Sensitive TRPA1 Ion Channel in Rat Urinary Bladder
Department of Pharmacology, Drug Development and Therapeutics, University of Turku, Turku, Finland, Department of Clinical Chemistry and Pharmacology, Lund University Hospital, Lund, Sweden.
Department of Clinical Chemistry and Pharmacology, Lund University Hospital, Lund, Sweden.
Linköping University, Department of Medical and Health Sciences, Clinical Pharmacology. Linköping University, Faculty of Health Sciences.
Wolfson Centre for Age-Related Diseases, King's College London, London, United Kingdom.
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2008 (English)In: European Urology, ISSN 0302-2838, E-ISSN 1873-7560, Vol. 53, no 2, 391-400 p.Article in journal (Refereed) Published
Abstract [en]

Objectives: To investigate the distribution of the transient receptor potential (TRP) A1 ion channel in the rat urinary bladder, and to study the effects of hydrogen sulfide (H2S) and known TRPA1 activators on micturition in conscious rats and on heterologously expressed ion channels. Methods: The expression of TRPA1 in urinary bladder was studied with fluorescence immunohistochemistry and real-time PCR in female Sprague-Dawley rats. Cystometric investigations were performed in conscious animals subjected to intravesical administration of sodium hydrogen sulfide (NaHS, donor of H2S), allyl isothiocyanate (AI), and cinnamaldehyde (CA). Fluorometric calcium imaging was used to study the effect of NaHS on human and mouse TRPA1 expressed in CHO cells. Results: TRPA1 immunoreactivity was found on unmyelinated nerve fibres within the urothelium, suburothelial space, and muscle layer as well as around blood vessels throughout the bladder. All TRPA1 immunoreactive nerves fibres also expressed TRPV1 immunoreactivity and vice versa. TRPA1 was also detected in urothelial cells at both transcriptional and protein levels. AI increased micturition frequency and reduced voiding volume. CA and NaHS produced similar changes in urodynamic parameters after disruption of the urothelial barrier with protamine sulfate. NaHS also induced calcium responses in TRPA1-expressing CHO cells, but not in untransfected cells. Conclusions: The expression of TRPA1 on C-fibre bladder afferents and urothelial cells together with the finding that intravesical TRPA1 activators initiate detrusor overactivity indicate that TRPA1 may have a role in sensory transduction in this organ. The study also highlights H2S as a TRPA1 activator potentially involved in inflammatory bladder disease. © 2007.

Place, publisher, year, edition, pages
2008. Vol. 53, no 2, 391-400 p.
Keyword [en]
Cystometry, Hydrogen sulfide, Micturition, Rat, Sensory neurons, Transient receptor potential ion channels, TRPA1, TRPV1, Urinary bladder, Urothelium
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-47133DOI: 10.1016/j.eururo.2007.10.024OAI: diva2:268029
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2012-05-24

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