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Quantification of adsorbed human serum albumin at solid interfaces: A comparison between radioimmunoassay (RIA) and simple null ellipsometry
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology.
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology.
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Applied Physics .
2000 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 18, no 2, 71-81 p.Article in journal (Refereed) Published
Abstract [en]

Radioimmunoassay (RIA) and null ellipsometry are two common methods to quantify adsorbed proteins. However, the accuracy of null ellipsometry with a constant protein refractive index (n=1.465, k=0) at ?=632.8 nm has this far not been explored. The present study compared the methods, and the degree of agreement between the simplified single wavelength null ellipsometry and RIA to quantify adsorbed proteins was explored on different surfaces. The quantification methods agreed well when Angstrom smooth hydrophilic or hydrophobic silicon surfaces, and freshly radio-labelled proteins were used. Some discrepancies were noted when either rough surface or stored and aged labelled proteins were used. The differences decreased when the aged protein solution was equilibrated with freshly dissolved proteins at room temperature (RT) for a few hours prior to the surface incubations. Significant differences were also noted between the methods when albumin was adsorbed at it's iso-electric point (pH 4.8). Copyright (C) 2000 Elsevier Science B.V.

Place, publisher, year, edition, pages
2000. Vol. 18, no 2, 71-81 p.
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-47606DOI: 10.1016/S0927-7765(99)00136-8OAI: oai:DiVA.org:liu-47606DiVA: diva2:268502
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2017-12-13

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Benesch, JohanAskendal, AgnetaTengvall, Pentti

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Engineering and Technology

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CiteExportLink to record
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  • Other style
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