Corticosteroids and interferons inhibit cytokine-induced production of IL-8 by human endothelial cells
2000 (English)In: Cytokine, ISSN 1043-4666, E-ISSN 1096-0023, Vol. 12, no 4, 355-360 p.Article in journal (Refereed) Published
IL-8, secreted by endothelial cells at the site of inflammation, participates in recruitment and transmigration of leukocytes. IL-8 may also have pathophysiological consequences in inflammatory and immunological disorders. We have investigated the effect of interferons (IFNs) and glucocorticosteroids (GCs) on cytokine induced secretion and production of IL-8 by human umbilical endothelial cells (HUVEC). There was a low spontaneous secretion of IL-8 by unstimulated HUVEC which increased after 6 or 24 h of stimulation with the pro-inflammatory cytokines TNF-α or IL-1β. IFN-γ as well as the GCs, Dexamethasone and Budesonide, inhibited TNF-α induced IL-8 secretion in a dose-dependent manner. IFNs may have a general modulating effect, since IFN-α also inhibited the TNF-α-induced IL-8 secretion. There was a slight, but significant, increase in the content of intracellular IL-8 in stimulated HUVEC. However, there was no difference between stimulation with IL-1β or TNF-α alone or in combination with IFNs or GCs, whereas inhibition of IL-8 secretion with monensin increased IL-8 content suggesting that IFNs and GCs inhibit synthesis rather than secretion of IL-8. In conclusion, IFNs or GCs may be useful for inhibiting IL-8 production by endothelial cells and could thus be used for therapeutic modulation of the inflammatory response.
Place, publisher, year, edition, pages
2000. Vol. 12, no 4, 355-360 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-47672DOI: 10.1006/cyto.1999.0557OAI: oai:DiVA.org:liu-47672DiVA: diva2:268568