Improved DNA flow cytometric, DNA ploidy, and S-phase reproducibility between 15 laboratories in analysis of breast cancer using generalized guidelines
2003 (English)In: Cytometry, ISSN 0196-4763, E-ISSN 1097-0320, Vol. 56A, no 1, 1-7 p.Article in journal (Refereed) Published
Lack of generalized guidelines for DNA flow cytometric analysis (FCM) may be the main reason for its limited use in the clinical management of breast cancer.
After an initial interlaboratory reproducibility study (Round I), we concluded that it was the evaluation of the DNA histograms rather than the technical performance of the analysis that was the main reason for discordant results between laboratories. Guidelines for the interpretation of DNA histograms were therefore drawn up. We present here data from a new reproducibility study (Round II) using these guidelines.
For 10 laboratories also participating in Round I, use of the guidelines increased the concordance in DNA ploidy status from 89% to 100% for the 46 samples used in both rounds. The concordance rate for SPF also increased; mean rs-value increased from 0.81 to 0.88, and mean kappa value (lower two-thirds versus upper third versus not reported) increased from 0.55 to 0.71. Five new laboratories, participating only in Round II, also agreed with the 10 original laboratories regarding DNA ploidy status. With the inclusion of all 15 laboratories, we obtained a mean rs-value of 0.81 and a mean kappa value of 0.72 for SPF.
Generalized guidelines for DNA FCM increase interlaboratory agreement, which is highly important in clinical routines and in multicenter studies. Furthermore, inexperienced FCM laboratories using generalized guidelines can produce and interpret DNA FCM data equally as well as experienced laboratories.
Place, publisher, year, edition, pages
2003. Vol. 56A, no 1, 1-7 p.
breast cancer, DNA flow cytometry, S-phase fraction, DNA-ploidy, reproducibility
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-47747DOI: 10.1002/cyto.a.10083OAI: oai:DiVA.org:liu-47747DiVA: diva2:268643