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Special ion effects: Why the properties of lysozyme in salt solutions follow a Hofmeister series
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Theoretical Physics .
2003 (English)In: Biophysical Journal, ISSN 0006-3495, Vol. 85, no 2, 686-694 p.Article in journal (Refereed) Published
Abstract [en]

Protein solubility in aqueous solutions depends in a complicated and not well understood way on pH, salt type, and salt concentration. Why for instance does the use of two different monovalent salts, potassium thiocyanate and potassium chloride, produce such different results? One important and previously neglected source of ion specificity is the ionic dispersion potential that acts between each ion and the protein. This attractive potential is found to be much stronger for SCN- than it is for Cl-. We present model calculations, performed within a modified ion-specific double-layer theory, that demonstrate the large effect of including these ionic dispersion potentials. The results are consistent with experiments performed on hen egg-white lysozymes and on neutral black lipid membranes. The calculated surface pH and net lysozyme charge depend strongly on the choice of anion. We demonstrate that the lysozyme net charge is larger, and the corresponding Debye length shorter, in a thiocyanate salt solution than in a chloride salt solution. Recent experiments have suggested that pK(a) values of histidines depend on salt concentration and on ionic species. We finally demonstrate that once ionic dispersion potentials are included in the theory these results can quantitatively be reinterpreted in terms of a highly specific surface pH (and a salt-independent pK(a)).

Place, publisher, year, edition, pages
2003. Vol. 85, no 2, 686-694 p.
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-48596OAI: diva2:269492
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2011-01-13

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Boström, Mathias
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