liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Ligand-specific targeting of microspheres to phagocytes by surface modification with poly(L-lysine)-grafted poly(ethylene glycol) conjugate
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
ETH, Dept Appl Biosci, Drug Formulat & Delivery Grp, CH-8057 Zurich, Switzerland Linkoping Univ, Dept Phys & Measurement Technol, Appl Phys Lab, SE-58183 Linkoping, Sweden Swiss Fed Inst Technol Zurich ETH, Dept Mech Engn, Biomicrometr Grp, CH-8952 Schlieren, Switzerland.
ETH, Dept Appl Biosci, Drug Formulat & Delivery Grp, CH-8057 Zurich, Switzerland Linkoping Univ, Dept Phys & Measurement Technol, Appl Phys Lab, SE-58183 Linkoping, Sweden Swiss Fed Inst Technol Zurich ETH, Dept Mech Engn, Biomicrometr Grp, CH-8952 Schlieren, Switzerland.
ETH, Dept Appl Biosci, Drug Formulat & Delivery Grp, CH-8057 Zurich, Switzerland Linkoping Univ, Dept Phys & Measurement Technol, Appl Phys Lab, SE-58183 Linkoping, Sweden Swiss Fed Inst Technol Zurich ETH, Dept Mech Engn, Biomicrometr Grp, CH-8952 Schlieren, Switzerland.
Show others and affiliations
2003 (English)In: Pharmaceutical research, ISSN 0724-8741, E-ISSN 1573-904X, Vol. 20, no 2, 237-246 p.Article in journal (Refereed) Published
Abstract [en]

Purpose. The purpose of this study was to demonstrate specific receptor- mediated targeting of phagocytes by functional surface coatings of microparticles, shielding from nonspecific phagocytosis and allowing ligand- specific interactions via molecular recognition. Methods. Coatings of the comb polymer poly( L- lysine)- g-poly( ethylene glycol) (PLL- g- PEG) were investigated for potential to inhibit 1) nonspecific spreading of human blood- derived macrophages (MOs) and dendritic cells (DCs) on glass and 2) nonspecific phagocytosis of PLL- g- PEG- coated, carboxylated polystyrene (PS) or biodegradable poly( D, L- lactide- co- glycolide) (PLGA) microspheres. Coating was performed by adsorption of positively charged PLL- g- PEG on negatively charged microparticles or plasma- cleaned glass through electrostatic interaction. The feasibility of ligand-specific interactions was tested with a model ligand, RGD, conjugated to PEG chains of PLL- g- PEG to form PLL- g- PEG- RGD and compared with inactive ligand conjugate, PLL- g- PEG- RDG. Results. Coatings with PLL- g- PEG largely impaired the adherence and spreading of MOs and DCs on glass. The repellent character of PLL- g- PEG coatings drastically reduced phagocytosis of coated PS and PLGA microparticles to 10% in presence of serum. With both MOs and DCs, we observed ligand- specific interactions with PLL- g-PEG- RGD coatings on glass and PS and PLGA microspheres. Ligand specificity was abolished when using inactive ligand conjugate PLL- g- PEG- RDG, whereas repellency of coating was maintained. Conclusions. Coatings of PLL- g- PEG- ligand conjugates provide a novel technology for ligand specific targeting of microspheres to MOs and DCs while reducing nonspecific phagocytosis.

Place, publisher, year, edition, pages
2003. Vol. 20, no 2, 237-246 p.
Keyword [en]
poly(D, L, -lactide-co-glycolide) (PLGA) microspheres, surface modification, poly(L-lysine)-grafted-poly(ethylene glycol) (PLL-g-PEG), phagocytosis, RGD-peptide
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-48687OAI: oai:DiVA.org:liu-48687DiVA: diva2:269583
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2017-12-12

Open Access in DiVA

No full text

By organisation
Department of Physics, Chemistry and BiologyThe Institute of Technology
In the same journal
Pharmaceutical research
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 38 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf