β-Carotene Uptake and Bioconversion to Retinol Differ Between Human Melanocytes and Keratinocytes
2001 (English)In: Nutrition and Cancer, ISSN 0163-5581, E-ISSN 1532-7914, Vol. 39, no 2, 300-306 p.Article in journal (Refereed) Published
β-Carotene is one of the carotenoids that has been considered to play a role in the natural defense against ultraviolet-induced skin cancer. It is not known whether epidermal cells are able to accumulate β-carotene and, subsequently, convert it to vitamin A. We used normal cultured human keratinocytes and melanocytes to study the uptake, and possible bioconversion to retinol, of authentic or [14C]β-carotene. The uptake was much higher in melanocytes than in keratinocytes, corresponding to a fivefold difference in the intracellular fraction after two days of incubation. An increased level of cellular retinol was noted after one day of β-carotene incubation. The conversion of [C]β-carotene to [14C]retinol peaked at 24 hours of incubation in keratinocytes and melanocytes. The results suggest that β-carotene can function as a local supply of vitamin A in the skin and that melanocytes are especially likely to store β-carotene.
Place, publisher, year, edition, pages
2001. Vol. 39, no 2, 300-306 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-49078DOI: 10.1207/S15327914nc392_21OAI: oai:DiVA.org:liu-49078DiVA: diva2:269974