Comparison of electron paramagnetic resonance methods to determine distances between spin labels on human carbonic anhydrase II
2001 (English)In: Biophysical Journal, ISSN 0006-3495, Vol. 80, no 6, 2886-2897 p.Article in journal (Refereed) Published
Four doubly spin-labeled variants of human carbonic anhydrase II and corresponding singly labeled variants were prepared by site-directed spin labeling. The distances between the spin labels were obtained from continuous-wave electron paramagnetic resonance spectra by analysis of the relative intensity of the half-field transition, Fourier deconvolution of line-shape broadening, and computer simulation of line-shape changes. Distances also were determined by four-pulse double electron-electron resonance. For each variant, at least two methods were applicable and reasonable agreement between methods was obtained. Distances ranged from 7 to 24 W. The doubly spin-labeled samples contained some singly labeled protein due to incomplete labeling. The sensitivity of each of the distance determination methods to the noninteracting component was compared.
Place, publisher, year, edition, pages
2001. Vol. 80, no 6, 2886-2897 p.
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-49243OAI: oai:DiVA.org:liu-49243DiVA: diva2:270139