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Regulation of Photosystem II core protein phosphorylation at the substrate level: Light induces exposure of the CP43 chlorophyll a protein complex to thylakoid protein kinase(s)
Stockholm Univ, Dept Biochem, Arrhenius Labs Nat Sci, S-10691 Stockholm, Sweden Linkoping Univ, Div Cell Biol, S-58185 Linkoping, Sweden Univ Munich, Inst Bot, D-80638 Munich, Germany Hebrew Univ Jerusalem, Dept Biol Chem, IL-91904 Jerusalem, Israel.
Stockholm Univ, Dept Biochem, Arrhenius Labs Nat Sci, S-10691 Stockholm, Sweden Linkoping Univ, Div Cell Biol, S-58185 Linkoping, Sweden Univ Munich, Inst Bot, D-80638 Munich, Germany Hebrew Univ Jerusalem, Dept Biol Chem, IL-91904 Jerusalem, Israel.
Stockholm Univ, Dept Biochem, Arrhenius Labs Nat Sci, S-10691 Stockholm, Sweden Linkoping Univ, Div Cell Biol, S-58185 Linkoping, Sweden Univ Munich, Inst Bot, D-80638 Munich, Germany Hebrew Univ Jerusalem, Dept Biol Chem, IL-91904 Jerusalem, Israel.
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2000 (English)In: Photosynthesis Research, ISSN 0166-8595, E-ISSN 1573-5079, Vol. 64, no 2-3, 209-219 p.Article in journal (Refereed) Published
Abstract [en]

Light induces conformational changes in the CP43 chl-a-protein antenna complex in isolated PS II core-complexes exposing phosphorylation site(s) to PS II core-associated protein kinase(s), to added solubilized thylakoid protein kinase(s), as well as to tryptic cleavage. The substrate-activation effect is demonstrated by exposure of the PS II cores to light during the kinase assay as well as by preillumination of the PS II cores in which the endogenous kinase(s) has been inactivated by treatment with N-ethylmaleimid. In the latter case, phosphorylation was performed in darkness following addition of the solubilized protein kinase(s). The solubilized protein kinase(s) does not require light activation. The apparent molecular masses of the main protein kinase(s) associated with the PS II cores (about 31-35 kDa and 45 kDa) differ from that of the major protein kinase present in solubilized preparations obtained from spinach thylakoids (64 kDa). The light-induced exposure of CP43 increases with the light intensity in the range of 20-100 mu mol photons m(-2) s(-1) as demonstrated by preillumination of N-ethylmaleimid treated cores followed by addition of the solubilized protein kinase(s) and performing the phosphorylation assay in darkness.

Place, publisher, year, edition, pages
2000. Vol. 64, no 2-3, 209-219 p.
Keyword [en]
conformational changes, CP43 complex, D1 protein, protein kinase, protein phosphorylation, phosphothreonine antibodies, PSII cores, trypsin cleavage
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-49389OAI: oai:DiVA.org:liu-49389DiVA: diva2:270285
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2017-12-12

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