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Bidirectional supply of glutamine maintains enterocyte ATP content in the in vitro Ussing chamber model
Linkoping Univ, Fac Hlth Sci, Dept Surg, S-58185 Linkoping, Sweden.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Surgery . Östergötlands Läns Landsting, Centre of Surgery and Oncology, Department of Surgery in Östergötland.
Linkoping Univ, Fac Hlth Sci, Dept Surg, S-58185 Linkoping, Sweden.
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2000 (English)In: International Journal of Colorectal Disease, ISSN 0179-1958, Vol. 15, no 5-6, 291-296 p.Article in journal (Refereed) Published
Abstract [en]

Glutamine is the principal energy source for enterocytes, but it is not known whether parenteral or enteral supplementation is most beneficial to gut integrity. The aim of this study was to evaluate the effects of glutamine in uni- or bidirectional supply on the viability of intestinal mucosa of starved rats during incubation in Ussing chambers. Segments of jejunum from rats starved for 48 h were randomly mounted in Ussing chambers with three nutrient solutions: Krebs buffer without glutamine, 6 mM glutamine added to the mucosal side, 6 mM glutamine added to the mucosal side and 0.6 mM glutamine to the serosal side. ATP content of the mucosa, electrophysiology, and Cr-51-ethyl-enediaminetetraacetate (EDTA) permeability were studied during 180 min of incubation. The addition of glutamine to both sides of the stripped mucosa improved ATP levels compared to the Krebs solution (P<0.05), and the addition of glutamine resulted in an increase in short circuit current (P<0.05). No significant differences were seen in Cr-51-EDTA permeability or epithelial electrical resistance. Glutamine supplementation to both the luminal and serosal side in the Ussing chamber was more effective than luminal glutamine only in maintaining ATP levels of intestinal mucosa. Bidirectional supplementation of glutamine might improve intestinal energy metabolism and viability in in vitro studies.

Place, publisher, year, edition, pages
2000. Vol. 15, no 5-6, 291-296 p.
Keyword [en]
Ussing chamber, glutamine, energy metabolism, intestinal mucosa, epithelial resistance, permeability
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-49493OAI: diva2:270389
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2011-01-14

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Söderholm, Johan DLindgren, Johan
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Faculty of Health SciencesSurgery Department of Surgery in Östergötland
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