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Can tissue drug concentrations be monitored by microdialysis during or after isolated limb perfusion for melanoma treatment?
Univ Queensland, Princess Alexandra Hosp, Dept Med, Brisbane, Qld 4109, Australia Univ Queensland, Princess Alexandra Hosp, Dept Surg, Brisbane, Qld 4109, Australia Linkoping Univ Hosp, Dept Dermatol, S-58185 Linkoping, Sweden.
Univ Queensland, Princess Alexandra Hosp, Dept Med, Brisbane, Qld 4109, Australia Univ Queensland, Princess Alexandra Hosp, Dept Surg, Brisbane, Qld 4109, Australia Linkoping Univ Hosp, Dept Dermatol, S-58185 Linkoping, Sweden.
Univ Queensland, Princess Alexandra Hosp, Dept Med, Brisbane, Qld 4109, Australia Univ Queensland, Princess Alexandra Hosp, Dept Surg, Brisbane, Qld 4109, Australia Linkoping Univ Hosp, Dept Dermatol, S-58185 Linkoping, Sweden.
2000 (English)In: Melanoma research, ISSN 0960-8931, E-ISSN 1473-5636, Vol. 10, no 1, 47-54 p.Article in journal (Refereed) Published
Abstract [en]

Isolated limb perfusion (ILP) with melphalan is used to treat recurrent melanoma. This study aimed to develop a microdialysis technique for melphalan tissue concentration measurement during ILP. The effects of melphalan concentration (50-600 mu g/ml), microdialysis flow rate (0.55-17.5 mu l/min), probe length (5-50 mm) and temperature (25-41.5 degrees C) on in vitro recovery were studied. In addition, in vivo recovery was measured in rat hindlimbs perfused with melphalan using 50 mm microdialysis probes implanted subcutaneously and into muscle. Both dialysate and tissue sample melphalan concentrations were determined by high performance liquid chromatography. The in vitro recovery of melphalan was not affected by melphalan concentration or temperature, but increased with probe length and decreased with flow rate. The melphalan concentrations in subcutaneous and muscle dialysates were not significantly different. A linear relationship was found between tissue dialysate concentrations and actual tissue concentrations of melphalan (r(2) = 0.97). Microdialysis is a potential method for tissue drug monitoring which may assist in the efficacious use of cytotoxics in human ILP. (C) 2000 Lippincott Williams & Wilkins.

Place, publisher, year, edition, pages
2000. Vol. 10, no 1, 47-54 p.
Keyword [en]
drug monitoring, isolated limb perfusion, microdialysis, tissue drug concentration
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-49835OAI: oai:DiVA.org:liu-49835DiVA: diva2:270731
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2017-12-12

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