liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
A designed branched three-helix bundle protein dimer
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology.
2006 (English)In: Journal of the American Chemical Society, ISSN 0002-7863, Vol. 128, no 22, 7287-7290 p.Article in journal (Refereed) Published
Abstract [en]

The ultimate goals of de novo protein design are the construction of novel tertiary structures and functions. Here is presented the design and synthesis of a uniquely branched three-helix bundle that folds into a well-folded dimeric protein. The branching of this protein was performed by the method of native chemical ligation, which provides a chemoselective and stable amide bond between the unprotected fragments. This ligation strategy was possible by the presented facile preparation of a peptide (43 amino acids) with a specific side chain thioester, which is synthesized by general Fmoc solid phase peptide synthesis. From the presented structural analysis, it is seen that the folded protein is present as a stable and highly helical dimer, thus forming a six-helix bundle. This unique tertiary structure, composed of a dimer of three individual a-helices branched together, offers different possibilities for protein engineering, such as metal and cofactor binding sites, as well as for the construction of novel functions. © 2006 American Chemical Society.

Place, publisher, year, edition, pages
2006. Vol. 128, no 22, 7287-7290 p.
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-50199DOI: 10.1021/ja060524kOAI: diva2:271095
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2011-01-11

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Dolphin, Gunnar
By organisation
The Institute of TechnologyDepartment of Physics, Chemistry and Biology
In the same journal
Journal of the American Chemical Society
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 27 hits
ReferencesLink to record
Permanent link

Direct link