Effects of CNI-1493 on human granulocyte functions
2006 (English)In: Immunobiology, ISSN 0171-2985, E-ISSN 1878-3279, Vol. 211, no 3, 191-197 p.Article in journal (Refereed) Published
During acute bacterial infections such as sepsis and meningitis, activation of inflammatory mediators such as nitric oxide (NO) plays a crucial role in both pathogenesis and host defense. We have previously reported that CNI-1493, a macrophage deactivator, reduced mortality in infant rats infected with Haemophilus influenzae type b (Hib) with associated decrease in the number of granulocytes in the infected tissue. The aim of the present study was to investigate how CNI-1493 affects granulocytes and macrophages in vitro. Murine macrophages (RAW 264.7) pre-incubated with CNI-1493 prior to activation with lipopolysaccharide (LPS)/interferon gamma (IFNγ) had decreased NO production measured as NO2−/NO3− levels and reduction in inducible NO-synthase (iNOS) expression. Reactive oxygen species (ROS) production was increased in formylmethionyl-leucyl-phenylalanine (FMLP)-stimulated granulocytes following CNI-1493 treatment, whereas F-actin content, motility and chemotaxis were decreased under the same conditions. The effects of CNI-1493 on both NO production in LPS/IFNγ-activated macrophages and ROS production, F-actin content, motility and chemotaxis in granulocytes, may contribute to the reduced inflammatory response and increased survival in Hib-infected animals treated with CNI-1493.
Place, publisher, year, edition, pages
2006. Vol. 211, no 3, 191-197 p.
CNI-1493, Granulocytes, iNOS, Macrophages, ROS
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-50239DOI: 10.1016/j.imbio.2005.09.006OAI: oai:DiVA.org:liu-50239DiVA: diva2:271135