A structural model of human steroid 11-betahydroxylase,CYP11B1, used to predict consequences of mutations
2009 (English)Article in journal (Other academic) Submitted
A prediction method has been developed to estimate the severity of amino acid residue exchanges in human steroid 11-beta-hydroxylase, CYP11B1, due to mutations in the corresponding gene. The prediction is based both on structural and on sequence dependent parameters. The method uses two approaches; one with general molecular property weights and one with a consensus voting strategy based upon distribution of molecular properties, which does not require any training. Both methods are tested on known mutations in CYP11B1 and result in 85% prediction accuracy. The consensus voting method is then further evaluated on 9 proteins with an average of 81% prediction accuracy. A server utilizing the results from the consensus voting on CYP11B1 is provided where the user can extract information about new mutants. A similar server is also provided for mutants in human steroid 21-hydroxylase (CYP21).
Place, publisher, year, edition, pages
CYP11B1, steroid 11-beta-hydroxylase, molecular modeling, structural prediction, mutations
National CategoryNatural Sciences
IdentifiersURN: urn:nbn:se:liu:diva-51118OAI: oai:DiVA.org:liu-51118DiVA: diva2:272971