Purification of human basophils by affinity chromatography on anti-IgE-sepharose 6MB
1981 (English)In: Allergy. European Journal of Allergy and Clinical Immunology, ISSN 0105-4538, Vol. 36, no 6, 411-417 p.Article in journal (Refereed) Published
This work describes a method for the purification of basophil leukocytes from human peripheral blood by the use of a three-step separation technique including affinity chromatography on anti-IgE-sepharose 6MB. The purity of the obtained basophils was 50--95% and the recovery was 30--40%. The basophils separated by this method appeared normal and were found to be reactive with anti-IgE in subsequent tests.
Place, publisher, year, edition, pages
1981. Vol. 36, no 6, 411-417 p.
Pharmacology and Toxicology
IdentifiersURN: urn:nbn:se:liu:diva-21021DOI: 10.1111/j.1398-9995.1981.tb01847.xPubMedID: 6172049OAI: oai:DiVA.org:liu-21021DiVA: diva2:275172