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Purification of human basophils by affinity chromatography on anti-IgE-sepharose 6MB
Linköping University, Faculty of Health Sciences. Linköping University, Department of Medicine and Care, Pharmacology.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Medicine and Care, Pharmacology.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Medicine and Care, Pharmacology.
1981 (English)In: Allergy. European Journal of Allergy and Clinical Immunology, ISSN 0105-4538, E-ISSN 1398-9995, Vol. 36, no 6, 411-417 p.Article in journal (Refereed) Published
Abstract [en]

This work describes a method for the purification of basophil leukocytes from human peripheral blood by the use of a three-step separation technique including affinity chromatography on anti-IgE-sepharose 6MB. The purity of the obtained basophils was 50--95% and the recovery was 30--40%. The basophils separated by this method appeared normal and were found to be reactive with anti-IgE in subsequent tests.

Place, publisher, year, edition, pages
1981. Vol. 36, no 6, 411-417 p.
Keyword [en]
basophils, purification
National Category
Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:liu:diva-21021DOI: 10.1111/j.1398-9995.1981.tb01847.xPubMedID: 6172049OAI: oai:DiVA.org:liu-21021DiVA: diva2:275172
Available from: 2009-11-03 Created: 2009-09-28 Last updated: 2017-12-12

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Toll, Johan BAndersson, Rolf G

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