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Neutralizing activity and cellular immune responses induced in mice after immunization with apoptotic HIV-1/murine leukemia virus infected cells
Linköping University, Department of Clinical and Experimental Medicine, Molecular Virology . Linköping University, Faculty of Health Sciences.
Karolinska University Hospital .
Lund University.
Karolinska University Hospital .
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2009 (English)In: VACCINE, ISSN 0264-410X, Vol. 27, no 46, 6424-6431 p.Article in journal (Refereed) Published
Abstract [en]

Dendritic cells present microbial antigens to T cells after uptake of apoptotic vesicles from infected cells. We previously reported that immunizations with apoptotic HIV-1/murine leukemia virus (MuLV) infected cells lead to induction of both cellular and humoral immune responses as well as resistance to mucosal challenge with live HIV-1/MuLV infected cells. Here we extended those studies and investigated whether apoptotic cells from HIV-1/MuLV infected cells stimulate the production of HIV-1 neutralizing activity. We compared different routes of administration and were able to induce p24- and Nef-specific cellular proliferation after intraperitoneal (i.p.), intranasal (i.n.), subcutaneous (s.c.) and intramuscular (i.m.) immunizations. Serum IgG and IgA antibodies directed against gp160, p24, or Nef were also produced regardless of immunization route used. However, the induction of mucosa-associated IgAs from faeces or vaginal secretions were detected only after either i.p. or i.n. immunizations. We were able to measure neutralizing activity in sera of mice after i.p. and i.n. immunization. Neutralizing reactivity was also detected after s.c. and i.m. immunizations in the presence of the cytokine adjuvant granulocyte macrophage-colony stimulating factor (GM-CSF). Conclusively we show induction of cellular and humoral immune responses including neutralizing activity after immunization with apoptotic HIV-1/MuLV infected cells in mice. The results from this study support further evaluations using apoptotic cells as antigen delivery system for vaccination against HIV-1 in other animal models.

Place, publisher, year, edition, pages
2009. Vol. 27, no 46, 6424-6431 p.
Keyword [en]
HIV-1, Virus neutralization, Apoptosis, T cells, B cells, Immunization routes
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-52391DOI: 10.1016/j.vaccine.2009.06.016OAI: oai:DiVA.org:liu-52391DiVA: diva2:282227
Note
Original Publication: Jorma Hinkula, Lilian Walther-Jallow, Anna Lauren, Barbro Makitalo, Monica Oberg, Britta Wahren, Eva-Maria Fenyo and Anna-Lena Spetz, Neutralizing activity and cellular immune responses induced in mice after immunization with apoptotic HIV-1/murine leukemia virus infected cells, 2009, VACCINE, (27), 46, 6424-6431. http://dx.doi.org/10.1016/j.vaccine.2009.06.016 Copyright: Elsevier Science B.V., Amsterdam. http://www.elsevier.com/ Available from: 2009-12-18 Created: 2009-12-18 Last updated: 2010-01-18

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