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Determination of in vivo Protein Phosphorylation in Photosynthetic Membranes
University of Turku, Turku, Finland.
Linköping University, Department of Clinical and Experimental Medicine, Cell Biology. Linköping University, Faculty of Health Sciences.
University of Turku, Turku, Finland.
2009 (English)In: Plant Signal Transduction: Methods and Protocols / [ed] Thomas Pfannschmidt, New York, NY, United States: Humana Press, 2009, Vol. 479, 133-146 p.Chapter in book (Other academic)
Abstract [en]

Light- and redox-controlled reversible phosphorylation of thylakoid proteins regulates short- and long-term acclimation of plants to environmental cues. The major phosphoproteins in thylakoids belong to photosystem II and its light-harvesting antenna but phosphorylation of subunits of other thylakoid protein complexes has been detected as well. The detection methods include electrophoretic separation of proteins and detection of phosphoproteins with a phosphoaminoacid-specific antibody or phosphoprotein-specific dye. The use of mass spectrometry allows the identification of exact phosphorylation site(s) in the proteins. Various methods for detection of phosphoproteins in thylakoids are outlined including phosphopeptide preparation for mass spectrometric analyses and quantitative analysis of protein phosphorylation.

Place, publisher, year, edition, pages
New York, NY, United States: Humana Press, 2009. Vol. 479, 133-146 p.
, Methods in Molecular Biology, ISSN 1064-3745 ; 479
Keyword [en]
Protein phosphorylation; phosphothreonine antibody; phosphospecific dye; mass spectrometry
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:liu:diva-52700DOI: 10.1007/978-1-59745-289-2_9ISBN: 978-1-58829-943-7 (print)ISBN: 978-1-59745-289-2 (online)OAI: diva2:284972
Available from: 2010-01-09 Created: 2010-01-09 Last updated: 2015-11-19Bibliographically approved

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Vener, Alexander
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Cell BiologyFaculty of Health Sciences
Biochemistry and Molecular Biology

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ReferencesLink to record
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