Determination of in vivo Protein Phosphorylation in Photosynthetic Membranes
2009 (English)In: Plant Signal Transduction: Methods and Protocols / [ed] Thomas Pfannschmidt, New York, NY, United States: Humana Press, 2009, Vol. 479, 133-146 p.Chapter in book (Other academic)
Light- and redox-controlled reversible phosphorylation of thylakoid proteins regulates short- and long-term acclimation of plants to environmental cues. The major phosphoproteins in thylakoids belong to photosystem II and its light-harvesting antenna but phosphorylation of subunits of other thylakoid protein complexes has been detected as well. The detection methods include electrophoretic separation of proteins and detection of phosphoproteins with a phosphoaminoacid-specific antibody or phosphoprotein-specific dye. The use of mass spectrometry allows the identification of exact phosphorylation site(s) in the proteins. Various methods for detection of phosphoproteins in thylakoids are outlined including phosphopeptide preparation for mass spectrometric analyses and quantitative analysis of protein phosphorylation.
Place, publisher, year, edition, pages
New York, NY, United States: Humana Press, 2009. Vol. 479, 133-146 p.
, Methods in Molecular Biology, ISSN 1064-3745 ; 479
Protein phosphorylation; phosphothreonine antibody; phosphospecific dye; mass spectrometry
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:liu:diva-52700DOI: 10.1007/978-1-59745-289-2_9ISBN: 978-1-58829-943-7 (print)ISBN: 978-1-59745-289-2 (online)OAI: oai:DiVA.org:liu-52700DiVA: diva2:284972