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G protein-coupled receptor mediated trimethylamine sensing
Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, The Institute of Technology.
Umea University, Department Mol Biol, SE-90187 Umea, Sweden .
2009 (English)In: BIOSENSORS and BIOELECTRONICS, ISSN 0956-5663, Vol. 25, no 4, 715-720 p.Article in journal (Refereed) Published
Abstract [en]

A new approach for the detection of trimethylamine (TMA) using a recombinant cell line of Xenopus laevis melanophores was developed. The cells were genetically modified to express the mouse trace amine-associated receptor 5 (mTAAR5), a G protein-coupled receptor from the mouse olfactory epithelium, which conferred high sensitivity to TMA. Cellular responses to TMA were analyzed by two different techniques, either by absorbance measurements using a microplate reader or by cellular imaging via an inverted microscope. A focused chemical screen allowed the discovery of additional, previously unknown stimuli of mTAAR5. The developed cell-based sensor demonstrated no sensitivity to trimethylamine N-oxide (TMAO), making it suitable for a straightforward evaluation of TMA levels in fish tissue extracts. For the detection of TMA vapor, the cells were covered with agarose, which allowed for intact cell viability for at least 6 h in air. The developed gas measurement platform was able to detect TMA from I to 100 ppm within 35 min.

Place, publisher, year, edition, pages
2009. Vol. 25, no 4, 715-720 p.
Keyword [en]
Cell-based sensor, Trace amine-associated receptor, TAAR5, Trimethylamine detection, Xenopus laevis melanophores
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-52835DOI: 10.1016/j.bios.2009.08.012OAI: oai:DiVA.org:liu-52835DiVA: diva2:285595
Available from: 2010-01-12 Created: 2010-01-12 Last updated: 2015-06-01

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Suska, AnkeBelen Ibanez, AnaLundström, Ingemar

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