Neuron survival in vitro is more influenced by the developmental age of the cells than by glucose condition
2009 (English)In: Cytotechnology (Dordrecht), ISSN 0920-9069, Vol. 61, no 1-2, 73-79 p.Article in journal (Refereed) Published
The objective of this study was to determine whether the sensitivity to varying glucose conditions differs for the peripheral and central nervous system neurons at different developmental stages. Ventral horn neurons (VHN) and dorsal root ganglion neurons (DRG) from rats of different postnatal ages were exposed to glucose-free or glucose-rich culture conditions. Following 24 h at those conditions, the number of protein gene product 9.5 positive (PGP(+)) DRG neurons and choline acetyltransferase positive (ChAT(+)) VHN were counted and their neurite lengths and soma diameters were measured. For both DRG and VHN, the highest number of cells with and without neurite outgrowth was seen when cells from postnatal day 4 donors were cultured, while the lowest cell numbers were when neurons were from donors early after birth and grown under glucose-free conditions. The length of the neurites and the soma diameter for VHN were not affected by either glucose level or age. DRG neurons, however, exhibited the shortest neurites and smallest soma diameter when neurons were obtained and cultured early after birth. Our results indicate that survival of neurons in vitro is more influenced by the developmental stage than by glucose concentrations.
Place, publisher, year, edition, pages
2009. Vol. 61, no 1-2, 73-79 p.
Age; DRG; Glucose; In vitro; Neurite outgrowth; Ventral horn neuron
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-52897DOI: 10.1007/s10616-009-9234-8OAI: oai:DiVA.org:liu-52897DiVA: diva2:285760
The original publication is available at www.springerlink.com:
Arian Sepehr, Johan Ruud and Simin Mohseni, Neuron survival in vitro is more influenced by the developmental age of the cells than by glucose condition, 2009, Cytotechnology (Dordrecht), (61), 1-2, 73-79.
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