liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Imaging phluorin-based probes at hippocampal synapses
School of Biomedical Sciences, University of Liverpool, Liverpool, UK.
MRC Laboratory of Molecular Biology, Cambridge, UK.
MRC Laboratory of Molecular Biology, Cambridge, UK.
MRC Laboratory of Molecular Biology, Cambridge, UK.
Show others and affiliations
2008 (English)In: Membrane Trafficking / [ed] Ales Vancura, Humana Press, 2008, Vol. 457, 293-303 p.Chapter in book (Other academic)
Abstract [en]

Accurate measurement of synaptic vesicle exocytosis and endocytosis is crucial to understanding the molecular basis of synaptic transmission. The fusion of a pH-sensitive green fluorescent protein (pHluorin) to various synaptic vesicle proteins has allowed the study of synaptic vesicle recycling in real time. Two such probes, synaptopHluorin and sypHy, have been imaged at synapses of hippocampal neurons in culture. The combination of these reporters with techniques for molecular interference, such as RNAi allows for the study of molecules involved in synaptic vesicle recycling. Here the authors describe methods for the culture and transfection of hippocampal neurons, imaging of pHluorin-based probes at synapses and analysis of pHluorin signals down to the resolution of individual synaptic vesicles.

Place, publisher, year, edition, pages
Humana Press, 2008. Vol. 457, 293-303 p.
, Methods in Molecular Biology (Methods and Protocols), ISSN 1064-3745 ; 457
Keyword [en]
Endocytosis; exocytosis; hippocampal synapses; imaging; neurons; synaptic vesicle; synaptopHluorin; sypHy
National Category
Bioinformatics (Computational Biology)
URN: urn:nbn:se:liu:diva-53183DOI: 10.1007/978-1-59745-261-8_22PubMedID: 19066036ISBN: 978-1-59745-261-8OAI: diva2:287563

The original publication is available at Stephen J Royle, Björn Granseth, Benjamin Odermatt, Aude Derevier and Leon Lagnado, Imaging phluorin-based probes at hippocampal synapses, 2008, Methods in Molecular Biology; Membrane Trafficking, (457), 293-303. Copyright: Humana Press

Available from: 2010-01-19 Created: 2010-01-19 Last updated: 2015-11-19Bibliographically approved

Open Access in DiVA

fulltext(808 kB)2134 downloads
File information
File name FULLTEXT03.pdfFile size 808 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedFind book in another country/Hitta boken i ett annat land

Search in DiVA

By author/editor
Granseth, Björn
Bioinformatics (Computational Biology)

Search outside of DiVA

GoogleGoogle Scholar
Total: 2139 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 169 hits
ReferencesLink to record
Permanent link

Direct link