Imaging phluorin-based probes at hippocampal synapses
2008 (English)In: Membrane Trafficking / [ed] Ales Vancura, Humana Press, 2008, Vol. 457, 293-303 p.Chapter in book (Other academic)
Accurate measurement of synaptic vesicle exocytosis and endocytosis is crucial to understanding the molecular basis of synaptic transmission. The fusion of a pH-sensitive green fluorescent protein (pHluorin) to various synaptic vesicle proteins has allowed the study of synaptic vesicle recycling in real time. Two such probes, synaptopHluorin and sypHy, have been imaged at synapses of hippocampal neurons in culture. The combination of these reporters with techniques for molecular interference, such as RNAi allows for the study of molecules involved in synaptic vesicle recycling. Here the authors describe methods for the culture and transfection of hippocampal neurons, imaging of pHluorin-based probes at synapses and analysis of pHluorin signals down to the resolution of individual synaptic vesicles.
Place, publisher, year, edition, pages
Humana Press, 2008. Vol. 457, 293-303 p.
, Methods in Molecular Biology (Methods and Protocols), ISSN 1064-3745 ; 457
Endocytosis; exocytosis; hippocampal synapses; imaging; neurons; synaptic vesicle; synaptopHluorin; sypHy
Bioinformatics (Computational Biology)
IdentifiersURN: urn:nbn:se:liu:diva-53183DOI: 10.1007/978-1-59745-261-8_22PubMedID: 19066036ISBN: 978-1-59745-261-8OAI: oai:DiVA.org:liu-53183DiVA: diva2:287563
The original publication is available at www.springerlink.com: Stephen J Royle, Björn Granseth, Benjamin Odermatt, Aude Derevier and Leon Lagnado, Imaging phluorin-based probes at hippocampal synapses, 2008, Methods in Molecular Biology; Membrane Trafficking, (457), 293-303. http://dx.doi.org/10.1007/978-1-59745-261-8 Copyright: Humana Press http://www.springer.com/2010-01-192010-01-192015-11-19Bibliographically approved