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A convenient and versatile hydroponic cultivation system for Arabidopsis thaliana
Stockholm University.
The Swedish University of Agricultural Sciences.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
2004 (English)In: Physiologia Plantarum: An International Journal for Plant Biology, ISSN 0031-9317, E-ISSN 1399-3054, Vol. 121, no 3, 343-348 p.Article in journal (Refereed) Published
Abstract [en]

A versatile two-step cultivation procedure for Arabidopsis thaliana is described for the production of large quantities of leaf material suitable for biochemical and biophysical analysis. The first step comprises a miniature greenhouse made out of a plastic pipette box to grow the seedlings to the six-leaf stage. For continued growth, the seedlings are transferred to hydroponic cultivation using an opaque container covered by a styrofoam lid. Transfer of the small seedlings to hydroponic culture is facilitated by growth in separate pipette tips, which protects vulnerable roots from damage. The hydroponic cultivation system is easy to scale-up and produces large amounts of relatively large leaves and roots. This hydroponic system produces enough plant material to make Arabidopsis a feasible model for biochemical and biophysical experiments, which can be combined with the available genetic information to address various aspects of plant functional genomics

Place, publisher, year, edition, pages
2004. Vol. 121, no 3, 343-348 p.
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-53639DOI: 10.1111/j.0031-9317.2004.00350.xOAI: oai:DiVA.org:liu-53639DiVA: diva2:290291
Available from: 2010-01-26 Created: 2010-01-26 Last updated: 2017-12-12

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Andersson, Bertil

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