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Sialic acid residues play a pivotal role in alpha(1)-acid glycoprotein (AGP)-induced generation of reactive oxygen species in chemotactic peptide pre-activated neutrophil granulocytes
Linköping University, Department of Medical and Health Sciences, Pharmacology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Medical and Health Sciences, Pharmacology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Cell Biology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Medical and Health Sciences, Pharmacology. Linköping University, Faculty of Health Sciences.
2010 (English)In: INFLAMMATION RESEARCH, ISSN 1023-3830, Vol. 59, no 2, 89-95 p.Article in journal (Refereed) Published
Abstract [en]

We have recently shown that terminal sialic acid residues are essential for alpha(1)-acid glycoprotein (AGP)-induced Ca2+ mobilization in neutrophils. The aim of the present study was to establish the importance of sialic acid residues on AGP in modulating human neutrophil functions, with emphasis on the generation of reactive oxygen species (ROS). ROS were measured by luminol-enhanced chemiluminescence in isolated human neutrophils. We found that AGP did not provoke ROS generation in resting or L-selectin presensitized neutrophils. Moreover, AGP did not affect the N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced ROS generation, but it slightly suppressed opsonized zymosan-induced responses. However, when the neutrophils were prestimulated with fMLP, the subsequent addition of AGP provoked a marked ROS response. Dose-response studies and time studies revealed that the ROS generating capacity of AGP was highest at a concentration of 0.05 mg/ml and when given 3-10 min after addition of fMLP. A desialylated form of AGP or pretreatment of neutrophils with 3- and 6-sialyllactose caused a substantially lower ROS response in neutrophils prestimulated with fMLP. Our data show that AGP can stimulate a second ROS response in fMLP preactivated neutrophils and that terminal sialic acid residues on AGP play a crucial role in this regard.

Place, publisher, year, edition, pages
2010. Vol. 59, no 2, 89-95 p.
Keyword [en]
alpha(1)-Acid glycoprotein, Neutrophils, Reactive oxygen species, Sialic acid
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-53823DOI: 10.1007/s00011-009-0071-1ISI: 000273787200001PubMedID: 19669698OAI: oai:DiVA.org:liu-53823DiVA: diva2:292219
Available from: 2010-02-05 Created: 2010-02-05 Last updated: 2014-08-27

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Gunnarsson, PeterFornander, LouisePåhlsson, PeterGrenegård, Magnus

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