Synthesis of a pentathiophene fluorescent probe, 4’,3’’’-Bis-carboxymethyl-[2,2’;5’,2’’;5’’,2’’’;5’’’,2’’’‘]quinquethiophene-5,5’
2010 (English)In: Nature Protocols, ISSN 1754-2189Article in journal (Other academic) Published
Poly and oligo-thiophenes have previously been used for in vitro, ex vivo and in vivo imaging of protein aggregates. The probe (p-FTAA) has been developed for the purpose of in vivo staining of protein aggregates such as amyloid deposits. It effectively passes the blood brain barrier and imaging can be performed live with two-photon imaging or ex vivo.
The straightforward synthesis of p-FTAA, including two Suzuki couplings, makes it an attractive probe for studies of most diseases involving protein aggregates.
Place, publisher, year, edition, pages
Nature Publishing Group , 2010.
Fluorescent probe, amyloid, prion, synthesis, oligothiophene
IdentifiersURN: urn:nbn:se:liu:diva-54045DOI: 10.1038/nprot.2010.24OAI: oai:DiVA.org:liu-54045DiVA: diva2:298014
ProjectsEfficient Imaging of Amyloid Deposits in Drosophila Models of Human Amyloidoses