DNA Chips with Conjugated Polyelectrolytes in Resonance Energy Transfer Mode
2010 (English)In: LANGMUIR, ISSN 0743-7463, Vol. 26, no 5, 3753-3759 p.Article in journal (Refereed) Published
We show how to use well-defined conjugated polyelectrolytes (CPEs) combined With Surface energy patterning to Fabricate DNA Chips utilizing A fluorescence signal amplification. Cholesterol-modified DNA strands in complex with it CPE are adsorbed to a surface energy pattern, formed by printing with soft elastomer stamps. Hybridization of the surface bound DNA strands with it short complementary strand from Solution is monitored using both fluorescence microscopy and imaging surface plasmon resonance. The CPEs act as antennas, enhancing resonance energy transfer to the dye-labeled DNA when complementary hybridization of the double strand occurs.
Place, publisher, year, edition, pages
2010. Vol. 26, no 5, 3753-3759 p.
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-54255DOI: 10.1021/la903101vISI: 000274636900113OAI: oai:DiVA.org:liu-54255DiVA: diva2:302292