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Development of a Point of Care Lateral Flow Device for Measuring Human Plasma Fibrinogen
Dublin City University.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Clinical Chemistry.
Dublin City University.
2010 (English)In: ANALYTICAL CHEMISTRY, ISSN 0003-2700, Vol. 82, no 5, 2029-2035 p.Article in journal (Refereed) Published
Abstract [en]

Fibrinogen is a major cardiovascular disease risk factor and is an independent predictor of coronary heart disease and stroke. Normal reference levels are approximately 2 to 4 g/L. Elevated levels are associated with the occurrence of cardiovascular disease-related events. The risk of increased bleeding in major surgery is inversely correlated with fibrinogen concentrations for concentrations below the upper limit of the reference interval, i.e., andlt;4 g/L. Determination of the clottable fibrinogen concentration in plasma is, thus, important for the investigation of coagulation disturbances in patients. A novel assay for monitoring plasma fibrinogen content has been developed on the basis of a simple, single use lateral flow microfluidic device. A 15 mu L plasma sample is applied to and travels along the microstructured device where it comes into contact with a thrombin reagent. This induces conversion of the soluble fibrinogen to insoluble fibrin and brings about clot formation. Lateral sample flow is arrested as a result of clotting, and the distance along the device at which this occurs has been shown to be dependent on the fibrinogen concentration. The test range was from 1 to 7 g/L of fibrinogen in undiluted patient plasma, and a result could be obtained within 5 min.

Place, publisher, year, edition, pages
2010. Vol. 82, no 5, 2029-2035 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-54408DOI: 10.1021/ac902763aISI: 000274841300064OAI: diva2:303517
Available from: 2010-03-12 Created: 2010-03-12 Last updated: 2010-03-12

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Lindahl, Tomas
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Clinical ChemistryFaculty of Health SciencesDepartment of Clinical Chemistry
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