Single-cell analysis of the common lymphoid progenitor compartment reveals functional and molecular heterogeneity
2010 (English)In: Blood, ISSN 0006-4971, E-ISSN 1528-0020, Vol. 115, no 13, 2601-2609 p.Article in journal (Refereed) Published
To investigate molecular events involved in the regulation of lymphoid lineage commitment, we crossed lambda 5 reporter transgenic mice to Rag1-GFP knockin mice. This allowed us to subfractionate common lymphoid progenitors and pre-pro-B (fraction A) cells into lambda 5(-)Rag1(low), lambda 5(-)Rag1(high), and lambda 5(+)Rag1(high) cells. Clonal in vitro differentiation analysis demonstrated that Rag1(low) cells gave rise to B/T and NK cells. Rag1(high) cells displayed reduced NK-cell potential with preserved capacity to generate B- and T-lineage cells, whereas the lambda 5(+) cells were B-lineage restricted. Ebf1 and Pax5 expression was largely confined to the Rag1high populations. These cells also expressed a higher level of the surface protein LY6D, providing an additional tool for the analysis of early lymphoid development. These data suggest that the classic common lymphoid progenitor compartment composes a mixture of cells with relatively restricted lineage potentials, thus opening new possibilities to investigate early hematopoiesis.
Place, publisher, year, edition, pages
American Society of Hematology , 2010. Vol. 115, no 13, 2601-2609 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-54870DOI: 10.1182/blood-2009-08-236398ISI: 000276201000009OAI: oai:DiVA.org:liu-54870DiVA: diva2:310843