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Accurate identification and quantification of 11-nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid in urine drug testing: Evaluation of a direct high efficiency liquid chromatographic-mass spectrometric method
Karolinska Institute.
National Board for Forensic Medicine.
Linköping University, Department of Medical and Health Sciences, Division of Drug Research. Linköping University, Faculty of Health Sciences.
Karolinska Institute.
2008 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 871, no 1, 101-108 p.Article in journal (Refereed) Published
Abstract [en]

A direct liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for measurement of urinary Delta(9)-tetrahydrocannabinol carboxylic acid (THCA) was developed. The method involved dilution of the urine sample with water containing H-2(9)-deuterated analogue as internal standard, hydrolysis with ammonia, reversed phase chromatography using a Waters ultra-performance liquid chromatography (UPLC (TM)) equipment with gradient elution, negative electrospray ionization, and monitoring of two product ions in selected reaction monitoring mode. The measuring range was 2-1000 ng/mL for THCA, and the intra- and inter-assay imprecision, expressed as the coefficient of variation, was below 5%. Influence from urine matrix on ionization efficiency was noted in infusion experiments, but was compensated for by the internal standard. Comparison with established gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry methods in authentic patient samples demonstrated accuracy in both qualitative and quantitative results. A small difference in mean ratios (similar to 15%) may be explained by the use of different hydrolysis procedures between methods. In conclusion, the high efficiency LC-MS/MS method was capable of accurately identify and quantify THCA in urine with a capacity of 14 samples per hour.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 2008. Vol. 871, no 1, 101-108 p.
Keyword [en]
urine; drug testing; (+/-)-11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol; LC-MS/MS
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-55092DOI: 10.1016/j.jchromb.2008.06.047ISI: 000258584100015OAI: diva2:315347
Available from: 2010-04-29 Created: 2010-04-29 Last updated: 2014-01-09Bibliographically approved

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Kronstrand, Robert
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ReferencesLink to record
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