liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
The creation of an antithrombotic surface by apyrase immobilization
Linnaeus University.
Linnaeus University.
University Uppsala Hospital.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Clinical Chemistry.
Show others and affiliations
2010 (English)In: BIOMATERIALS, ISSN 0142-9612, Vol. 31, no 16, 4484-4491 p.Article in journal (Refereed) Published
Abstract [en]

Blood incompatibility reactions caused by surfaces often involve platelet activation and subsequent platelet-initiated activation of the coagulation and complement cascades. The goal of this study was to immobilize apyrase on a biomaterial surface in order to develop an enzymatically active surface that would have the capacity to inhibit platelet activation by degrading ADP. We were able to immobilize apyrase on a polystyrene surface with preservation of the enzymatic activity. We then analyzed the hemocompatibility of the apyrase surface and of control surfaces by incubation with platelet-rich plasma (PRP) or whole blood. Monitoring of markers of platelet, coagulation, and complement activation and staining of the surfaces revealed decreased levels of platelet and coagulation activation parameters on the apyrase surface. The formation of antithrombin-thrombin and antithrombin-factor XIa complexes and the extent of platelet consumption were significantly lower on the apyrase surface than on any of the control surfaces. No significant differences were seen in complement activation (C3a levels). Staining of the apyrase surface revealed low platelet adherence and no formation of granulocyte platelet complexes. These results demonstrate that it is possible to create an antithrombotic surface targeting the ADP amplification of platelet activation by immobilizing apyrase.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 2010. Vol. 31, no 16, 4484-4491 p.
Keyword [en]
Blood compatibiliy, Apyrase, Platelet, Platelet regulation, Adenosine diphosphate
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-56692DOI: 10.1016/j.biomaterials.2010.02.036ISI: 000277549600004OAI: diva2:321228
Available from: 2010-05-31 Created: 2010-05-31 Last updated: 2010-05-31

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Faxälv, LarsLindahl, Tomas
By organisation
Clinical ChemistryFaculty of Health SciencesDepartment of Clinical Chemistry
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 19 hits
ReferencesLink to record
Permanent link

Direct link