liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Improved forensic DNA analysis through the use of alternative DNA polymerases and statistical modeling of DNA profiles
Lund University.
Linköping University, Department of Computer and Information Science, Statistics. Linköping University, Faculty of Arts and Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Medical Microbiology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Physics, Chemistry and Biology, Molecular genetics. Linköping University, Faculty of Health Sciences.
Show others and affiliations
2009 (English)In: BIOTECHNIQUES, ISSN 0736-6205, Vol. 47, no 5, 951-958 p.Article in journal (Refereed) Published
Abstract [en]

DNA evidence, linking perpetrators to crime scenes, is central to many legal proceedings. However, DNA samples from crime scenes often contain PCR-inhibitory substances, which may generate blank or incomplete DNA profiles. Extensive DNA purification can be required to rid the sample of these inhibitors, although these procedures increase the risk of DNA loss. Most forensic laboratories use commercial DNA amplification kits (e.g., AmpFlSTR SGM Plus) with the DNA polymerase AmpliTaq Gold as the gold standard. Here, we show that alternative DNA polymerase-buffer systems can improve the quality of forensic DNA analysis and efficiently circumvent PCR inhibition in crime scene samples, without additional sample preparation. DNA profiles from 20 of 32 totally or partially inhibited crime scene saliva samples were significantly improved using Bio-X-Act Short, ExTaq Hot Start, or PicoMaxx High Fidelity instead of AmpliTaq Gold. A statistical model for unbiased quality control of forensic DNA profiles was developed to quantify the results. Our study demonstrates the importance of adjusting the chemistry of the PCR to enhance forensic DNA analysis and diagnostic PCR, providing an alternative to laborious sample preparation protocols.

Place, publisher, year, edition, pages
Eaton Publishing , 2009. Vol. 47, no 5, 951-958 p.
Keyword [en]
crime scene samples, DNA polymerase, forensic DNA analysis, PCR inhibition, principal components, statistical modeling
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-56691DOI: 10.2144/000113246ISI: 000277560200010OAI: oai:DiVA.org:liu-56691DiVA: diva2:321229
Available from: 2010-05-31 Created: 2010-05-31 Last updated: 2012-01-17

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Nordgaard, AndersRasmusson, BirgittaAnsell, Ricky

Search in DiVA

By author/editor
Nordgaard, AndersRasmusson, BirgittaAnsell, Ricky
By organisation
StatisticsFaculty of Arts and SciencesMedical MicrobiologyFaculty of Health SciencesMolecular genetics
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 146 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf