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In Vitro Study of Recruitment Ability of Macrophages and Trophoblasts in Early Human Pregnancy
Linköping University, Department of Physics, Chemistry and Biology.
2010 (English)Independent thesis Advanced level (degree of Master (Two Years)), 40 credits / 60 HE creditsStudent thesis
Abstract [en]

The tolerance towards the semi-allogenic foetus is obtained through both systemic and local changes in the maternal immune response. Locally, in the decidua, the cell composition differs from that found in the blood; natural killer (NK) cells and macrophages being the major cell types. Decidual macrophages (dMØ), which are alternatively activated, and trophoblasts, placental cells of foetal origin, are believed to participate in the foetal tolerance at the foetal-maternal interface. To test the recruitment ability of macrophages and trophoblasts, and to test if these cells are responsible for the special cell composition in the decidua, a migration assay was established. In this migration assay peripheral blood mononuclear cells (PBMC) were allowed to migrate through Matrigel-coated transwell inserts into lower wells containing a recruiting stimulus. After testing several conditions, a protocol was established for further use. The results showed that in vitro alternatively activated macrophages, which display many of the surface markers as dMØ, hold a recruiting ability and recruit monocytes. Further there was an indication that trophoblasts also hold a recruiting ability. Neither cell types were shown to recruit NK cells. In conclusion, this study presents a suitable protocol for assessing chemotactic factors and different cell type’s ability to recruit cells from blood. Although the experiments need to be repeated and extended and the recruitment ability of dMØ needs to be evaluated in detail before a final conclusion can be drawn, the preliminary data indicated that macrophages and trophoblasts can recruit monocytes.

Place, publisher, year, edition, pages
2010. , 31 p.
Keyword [en]
Decidua, macrophages, Matrigel, migration assay, pregnancy, trophoblasts
National Category
Immunology in the medical area
Identifiers
URN: urn:nbn:se:liu:diva-56818ISRN: LITH-IFM-A-EX--10/2313--SEOAI: oai:DiVA.org:liu-56818DiVA: diva2:322330
Presentation
2010-05-28, Linköpings universitet, 15:39 (English)
Uppsok
Life Earth Science
Supervisors
Examiners
Available from: 2010-06-29 Created: 2010-06-04 Last updated: 2011-03-24Bibliographically approved

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Masters thesis - Caroline Wendel(1095 kB)814 downloads
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CiteExportLink to record
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Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
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  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
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  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
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Output format
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  • rtf