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Analysis of extracellular matrix proteins produced by cultured cells
Institute of Cytology RAS, St. Petersburg, Russian Federation.
Institute of Cytology RAS, St. Petersburg, Russian Federation.
Institute of Cytology RAS, St. Petersburg, Russian Federation.
Linköping University, Department of Clinical and Experimental Medicine, Medical Microbiology. Linköping University, Faculty of Health Sciences.
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2009 (Russian)In: Tsitologiya, ISSN 0041-3771, Vol. 51, no 8, 691-696 p.Article in journal (Refereed) Published
Abstract [en]

Extracellular matrix (ECM) is a highly organized multimolecular structure essential for vital function of any organism. Although a lot of data on the extracellular matrix components has been accumulated, an isolation of the entire set of these proteins still remains to be a complex procedure since ECM contains fibrillar proteins and proteoglycans, which form multidomain net-like structures. In the presented study, we developed a method for isolation of ECM proteins from cell cultures. Human epidermoid carcinoma cells A431 and fibroblasts obtained from normal and scar human skin were used. We showed that EDTA solution removed cells from culture plates without destroying the cell membrane. Following treatment of remaining ECM proteins with acetic acid in order to dissociate collagen fibrils significantly improved fractioning of ECM proteins. Extraction of the remained proteins from culture plate surface was preformed using a buffer developed on the basis of Laemmli probe buffer. With this method, we isolated ECM proteins synthesized by culturing cells and suitable for a future analysis by SDS PAGE and two-dimentional electrophoresis as well as for identification of individual proteins by mass-spectrometry. This study may allow comparing protein contents of ECMs isolated from different sources, and elucidate influences of various proteins on the protein and the properties of extracellular matrix of investigated cells.

Place, publisher, year, edition, pages
St Petersburg, Russian Federation: Sankt-Peterburgskaya Izdatel'skaya Firma Nauka , 2009. Vol. 51, no 8, 691-696 p.
Keyword [en]
Collagen; Extracellular matrix; Fibroblasts; Fibronectin
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:liu:diva-57044PubMedID: 19799354Scopus ID: 2-s2.0-70449727082OAI: oai:DiVA.org:liu-57044DiVA: diva2:323989
Note

Englisg version of this article is available at http://link.springer.com/article/10.1134%2FS1990519X09050137

Available from: 2010-06-14 Created: 2010-06-09 Last updated: 2017-01-19Bibliographically approved

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PubMedScopusLink to article/lLänk till artikel (Russian/Ryska)http://link.springer.com/article/10.1134%2FS1990519X09050137

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Magnusson, Karl-Eric

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