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Analysis of nuclear protein complexes comprising a-actinin-4 by 2D-electrophoresis and mass-spectrometry
Institute of Cytology RAS, St. Petersburg, Russian Federation.
Institute of Cytology RAS, St. Petersburg, Russian Federation.
Institute for Analytical Instrumentation RAS, St. Petersburg, Russian Federation.
Institute for Analytical Instrumentation RAS, St. Petersburg, Russian Federation.
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2009 (English)In: Tsitologiya, ISSN 0041-3771, Vol. 51, no 8, 684-690 p.Article in journal (Refereed) Published
Abstract [en]

Actin-binding protein a-actinin-4 is a member of spectrin super family. It is located in the cytoplasm and in the nucleus. However, nuclear functions of a-actinin-4 are still not clear. In this study, we analyzed composition of nuclear protein complexes associated with a-actinin-4 in A431 cells. Using 2D electrophoresis, we have determined that about 50 different proteins may be associated with nuclear a-actinin-4. Using mass-spectrometry, we analyzed major proteins of these complexes. ß-Actin, a- and ß-tubulins, ribonucleoprotein A2/B1, which regulates splicing and is associated with ß-actin, peroxiredoxin-1, which is involved in oxidative stress, and glycolytic enzyme D-3-phosphoglycerate dehydrogenase were identified by MALDI-TOF. Detection of these proteins in nuclear complexes with a-actinin-4 may suggest that a-actinin-4 is involved in transcription and splicing. Presence of a-actin in the investigated complexes was confirmed by tandem mass-spectrometry (MALDITOF-TOF). Immunoprecipitation of nuclear proteins with antibodies against a-tubulin confirmed association of a-actinin-4 with a-tubulin in the protein complex. Nuclear a-actinin-4 constitutes of 105 KDa fullsize isoform and two truncated isoforms of 65 and 75 kDa, whereas only the truncated isoform have been found in nuclear complexes with a-tubulin. These data suggest that a-actinin-4 is associated with a number of different nuclear protein complexes which may carry out different functions in the cell nucleus.

Place, publisher, year, edition, pages
SP MAIK Nauka/Interperiodica , 2009. Vol. 51, no 8, 684-690 p.
Keyword [en]
a-actinin-4; Mass-spectrometry
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-57041DOI: 10.1134/S1990519X09050058OAI: oai:DiVA.org:liu-57041DiVA: diva2:323996
Available from: 2010-06-14 Created: 2010-06-09 Last updated: 2014-08-27

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Magnusson, Karl-Eric

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