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Plasmonic Enhanced Fluorescence using Gold Nanorods
Linköping University, Department of Physics, Chemistry and Biology.
2010 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

The aims of this study are to first immobilize positively charged gold nanorods to negatively charged cell culture surfaces. Second, to use polyelectrolytes for controlling the distance between gold nanorods and fluorophores. This is used to optimally determine the distance, of which maximum fluorescence enhancement is achieved, between gold nanorods and fluorophores. In order to approach these aims, we use UV/VIS absorption spectroscopy, fluorescence spectroscopy, atomic force microscopy, and ellipsometry. The results show that we could control the immobilization of gold nanorods on plastic microwell plates and create reproducible polyelectrolyte layers, in order to control the distance between the gold nanorods and fluorophores. In addition, the localized surface plasmon resonance wavelength red shifted as the PELs increased. In conclusion, we found that the maximum fluorescence enhancement of the fluorophores (Cy7) is about 2.3 times at a fluorophores-nanoparticles separation of approximately 9-12 nm. This work contributes some research information towards the design of optical biochip platforms based on plasmon-enhanced fluorescence.

Place, publisher, year, edition, pages
2010. , 49 p.
Keyword [en]
Localized Surface Plasmon Resonance, plasmonic gold nanorods, Polyelectrolyte, Layer-by- Layer, Fluorophore, Plasmonic Enhanced Fluorescence, Microwell Plate
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-57680ISRN: LITH-IFM-A-EX--10/2298—SEOAI: oai:DiVA.org:liu-57680DiVA: diva2:327299
Presentation
2010-06-24, seminar room Röntgen (P404), IFM · Linköpings universitet · 581 83 LINKÖPING, Sweden, 09:00 (English)
Uppsok
Physics, Chemistry, Mathematics
Supervisors
Examiners
Available from: 2010-07-05 Created: 2010-06-28 Last updated: 2010-07-05Bibliographically approved

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CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf